Anti-Eph receptor A4 antibody (ab7039)

Overview

  • Product nameAnti-Eph receptor A4 antibody
    See all Eph receptor A4 primary antibodies
  • Description
    Goat polyclonal to Eph receptor A4
  • SpecificityThe antibody will recognize recombinant mouse EphA4 by immunoblotting and ELISA. By immunoblotting and ELISA, the antibody shows approximately 10% cross-reactivity with recombinant human Ephrin-A5 and <5% cross-reactivity with recombinant human EphA1, mouse EphA2, mouse EphA3, rat EphA5, mouse EphA6, mouse EphA7 and mouse EphA8.
  • Tested applicationsSuitable for: WB, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant fragment (extracellular domain) (Mouse). Expressed in NSO cells.

  • Positive control
    • recombinant protein
  • General notes


    EphA4 (the antigen to this antibody) is also known Sek, Sek1, Cek8, Hek8 and Tyro1 and binds to ephrin-A5, ephrin-A1, ephrin-A3, ephrin-A2, ephrin-B2, ephrin-B3 and ephrin-A4. EphA4 can be detected in B cells, T cells and keratinocytes (ref 1,2). To date, at least 14 members of the Eph receptor family and a family of 8 ligands have been identified. Ligands of Eph family receptors are structurally related membrane-bound proteins that can be subdivided into two major subclasses: ephrin-A and ephrin-B (ref 2). Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The a general role of the Eph family is in mediating repulsive cell-cell interaction, as suggested by studies of axonal guidance and neural crest cell migration.

Properties

Applications

Our Abpromise guarantee covers the use of ab7039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.

WB (colourimetric): Use at a concentration of 0.1 - 0.2 µg/ml.

Indirect ELISA Use a concentration of 0.5 - 1 µg/ml.

Target

  • FunctionReceptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in a signal transduction process involved in hindbrain pattern formation.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • DomainThe protein kinase domain mediates interaction with NGEF/ephexin-1.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cek 8 antibody
    • CEK8 antibody
    • EK8 antibody
    • eph receptor a4 antibody
    • EPH-like kinase 8 antibody
    • EPHA4 antibody
    • EPHA4_HUMAN antibody
    • Ephrin type-A receptor 4 antibody
    • HEK 8 antibody
    • hEK8 antibody
    • Receptor protein-tyrosine kinase HEK8 antibody
    • Sek 1 antibody
    • SEK antibody
    • TYRO 1 protein tyrosine kinase antibody
    • TYRO1 antibody
    • Tyrosine-protein kinase receptor SEK antibody
    • Tyrosine-protein kinase TYRO1 antibody
    see all

References for Anti-Eph receptor A4 antibody (ab7039)

ab7039 has not yet been referenced specifically in any publications.

Product Wall

The crossreactivity of this Ab with Ephrin A5 was based on Purification test data, and the estimation was accurate. QC did not test its crossreactivity with mEphrin A5, because mEphrin A5 was not listed as a crossreactant for mEpHA4 Ab. The immunoge...

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