Recombinant fragment (extracellular domain) (Mouse). Expressed in NSO cells.
EphA4 (the antigen to this antibody) is also known Sek, Sek1, Cek8, Hek8 and Tyro1 and binds to ephrin-A5, ephrin-A1, ephrin-A3, ephrin-A2, ephrin-B2, ephrin-B3 and ephrin-A4. EphA4 can be detected in B cells, T cells and keratinocytes (ref 1,2). To date, at least 14 members of the Eph receptor family and a family of 8 ligands have been identified. Ligands of Eph family receptors are structurally related membrane-bound proteins that can be subdivided into two major subclasses: ephrin-A and ephrin-B (ref 2). Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The a general role of the Eph family is in mediating repulsive cell-cell interaction, as suggested by studies of axonal guidance and neural crest cell migration.
Our Abpromise guarantee covers the use of ab7039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.
WB (colourimetric): Use at a concentration of 0.1 - 0.2 µg/ml.
|Indirect ELISA||Use a concentration of 0.5 - 1 µg/ml.|
ab7039 has not yet been referenced specifically in any publications.