Overview

  • Product nameAnti-Eph receptor A5 antibody
    See all Eph receptor A5 primary antibodies
  • Description
    Rabbit polyclonal to Eph receptor A5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within internal sequence amino acids 791-840 (SDMGYVHRDL AARNILINSN LVCKVSDFGL SRVLEDDPEA AYTTRGGKIP) of Human Eph receptor A5 (NP_004430).

  • Positive control
    • Human fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 115 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionReceptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. Beside its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion.
  • Tissue specificityAlmost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 1 Eph LBD (Eph ligand-binding) domain.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation is stimulated by the ligand EFNA5. Dephosphorylation upon stimulation by glucose, inhibits EPHA5 forward signaling and results in insulin secretion.
  • Cellular localizationCell membrane. Cell projection > axon. Cell projection > dendrite.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain-specific kinase antibody
    • bsk antibody
    • CEK 7 antibody
    • Eck like sequence 1 antibody
    • EHK 1 antibody
    • EHK-1 antibody
    • EK7 antibody
    • Els 1 antibody
    • EPH homology kinase 1 antibody
    • EPH-like kinase 7 antibody
    • EPHA5 antibody
    • EPHA5_HUMAN antibody
    • Ephrin type A receptor 5 antibody
    • Ephrin type-A receptor 5 antibody
    • HEK 7 antibody
    • hEK7 antibody
    • Receptor protein tyrosine kinase HEK 7 antibody
    • Rek 7 antibody
    • TYRO 4 antibody
    • Tyrosine protein kinase receptor EHK 1 antibody
    see all

Anti-Eph receptor A5 antibody images

  • Anti-Eph receptor A5 antibody (ab85645) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 115 kDa
    Observed band size : 115 kDa

References for Anti-Eph receptor A5 antibody (ab85645)

ab85645 has not yet been referenced specifically in any publications.

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