The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesDot: Use at a concentration of 1 - 10 µg/ml using peroxidase conjugated anti-sheep IgG and detection by chemiluminescence.
IP: Use at a concentration of 10 - 20 µg/ml.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. Binding with the guidance cue ephrin-B2 at the optic chiasm midline redirect ventrotemporal (VT) retinal ganglion cells (RGCs) axons ipsilaterally. May be involved in cell-cell interactions in the nervous system.
Tissue specificityPreferentially expressed in brain.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.