Synthesized phosphopeptide derived from human EPHB1 around the phosphorylation site of tyrosine 594 (K-I-YP-I-D).
Our Abpromise guarantee covers the use of ab61791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).|
|ICC/IF||1/500 - 1/1000.|
This image is courtesy of an Abreview submitted by Thomas Jungas
All cells were stimulated with preclustered ephrinb1-Fc chimeric protein.
ab61791 staining Eph receptor B1 + Eph receptor B2 (phospho Y594) in HeLa cells (overexpressing EphB2 and stimulated for 20 minutes with preclustered efnb1-Fc chimeric protein) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 0.1% Triton X-100) for 2 hour at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody. Red - anti-EphB2 (goat), Grey - DAPI, Green - ab61791.