The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. PubMed: 20496368
Use a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant mouse EphB3 is approx. 0.6 ng/well.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 82 kDa. The detection limit for recombinant mouse EphB3 is approximately 5 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. As a result of glycosylation, recombinant EphB3/Fc migrates as an approximately 100 kDa protein under reducing conditions in SDS-PAGE.
FunctionReceptor for members of the ephrin-B family. Binds to ephrin-B1 and -B2.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.
References for Anti-Eph receptor B3 antibody (ab10617)
This product has been referenced in:
Theus MH et al. EphB3 Limits the Expansion of Neural Progenitor Cells in the Subventricular Zone by Regulating p53 During Homeostasis and Following Traumatic Brain Injury. Stem Cells28:1231-42 (2010).
Read more (PubMed: 20496368) »