Anti-Eph receptor B4 antibody (ab93902)


  • Product nameAnti-Eph receptor B4 antibody
    See all Eph receptor B4 primary antibodies
  • Description
    Rabbit polyclonal to Eph receptor B4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human Eph receptor B4.

  • Positive control
    • This antibody gave a positive signal in both Human placenta tissue lysate and A549 whole cell lysate.



Our Abpromise guarantee covers the use of ab93902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 108 kDa).

Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product.


  • FunctionReceptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development.
  • Tissue specificityAbundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ephb4 antibody
    • EPHB4_HUMAN antibody
    • Ephrin receptor EphB4 antibody
    • Ephrin type-B receptor 4 antibody
    • Hepatoma Transmembrane Kinase antibody
    • HTK antibody
    • MYK 1 antibody
    • MYK1 antibody
    • TYRO 11 antibody
    • TYRO11 antibody
    • Tyrosine-protein kinase receptor HTK antibody
    • Tyrosine-protein kinase TYRO11 antibody
    see all

Anti-Eph receptor B4 antibody images

  • All lanes : Anti-Eph receptor B4 antibody (ab93902) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 108 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

    The predicted molecular weight of Eph receptor B4 is 108 kDa (SwissProt), however we expect to observe a banding pattern at 125 kDa. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References for Anti-Eph receptor B4 antibody (ab93902)

ab93902 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50000 cells
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (A549, HEK293)
Specification A549, HEK293
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 02 2014