Anti-Eph receptor B4 antibody [RM0056-6A14] (ab73259)

Overview

  • Product nameAnti-Eph receptor B4 antibody [RM0056-6A14]
    See all Eph receptor B4 primary antibodies
  • Description
    Rat monoclonal [RM0056-6A14] to Eph receptor B4
  • Specificityab73259 is specific for mouse Eph receptor B4. It does not cross-react with recombinant mouse EphA2, EphA3, EphA4, EphA6, EphA7 and EphB6
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant mouse Eph receptor B4 extracellular domain

Properties

Applications

Our Abpromise guarantee covers the use of ab73259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 109 kDa.
IHC-P 1/100 - 1/200.

Target

  • FunctionReceptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development.
  • Tissue specificityAbundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ephb4 antibody
    • EPHB4_HUMAN antibody
    • Ephrin receptor EphB4 antibody
    • Ephrin type-B receptor 4 antibody
    • Hepatoma Transmembrane Kinase antibody
    • HTK antibody
    • MYK 1 antibody
    • MYK1 antibody
    • TYRO 11 antibody
    • TYRO11 antibody
    • Tyrosine-protein kinase receptor HTK antibody
    • Tyrosine-protein kinase TYRO11 antibody
    see all

Anti-Eph receptor B4 antibody [RM0056-6A14] images

  • ab73259 at 1/200 dilution, staining Eph receptor B4 in LPS treated mouse kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • ab73259 staining the Eph receptor B4 in Mouse lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was not performed. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-rat polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-Eph receptor B4 antibody [RM0056-6A14] (ab73259)

ab73259 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lymph node)
Specification Lymph node
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Submitted Sep 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"