Anti-Eph receptor B6 antibody (ab54656)

Overview

  • Product nameAnti-Eph receptor B6 antibody
    See all Eph receptor B6 primary antibodies
  • Description
    Mouse monoclonal to Eph receptor B6
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: DTTGETSEIG WLTYPPGGWD EVSVLDDQRR LTRTFEACHV AGAPPGTGQD NWLQTHFVER RGAQRAHIRL HFSVRACSSL GVSGGTCRET FTLYYRQAEE , corresponding to amino acids 23-122 of Human Eph receptor B6

Properties

Applications

Our Abpromise guarantee covers the use of ab54656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionKinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2.
    • Tissue specificityExpressed in brain. Expressed in non invasive breast carcinoma cell lines (at protein level). Strong expression in brain and pancreas, and weak expression in other tissues, such as heart, placenta, lung, liver, skeletal muscle and kidney. Expressed in breast non invasive tumors but not in metastatic lesions. Isoform 3 is expressed in cell lines of glioblastomas, anaplastic astrocytomas, gliosarcomas and astrocytomas. Isoform 3 is not detected in normal tissues.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 1 protein kinase domain.
      Contains 1 SAM (sterile alpha motif) domain.
    • DomainThe protein kinase domain is predicted to be catalytically inactive. Its extracellular domain is capable of promoting cell adhesion and migration in response to low concentrations of ephrin-B2, but its cytoplasmic domain is essential for cell repulsion and inhibition of migration induced by high concentrations of ephrin-B2.
    • Post-translational
      modifications
      Ligand-binding increases phosphorylation on tyrosine residues. Phosphorylation on tyrosine residues is mediated by transphosphorylation by the catalytically active EPHB1 in a ligand-independent manner. Tyrosine phosphorylation of the receptor may act as a switch on the functional transition from cell adhesion/attraction to de-adhesion/repulsion.
    • Cellular localizationSecreted and Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • EPH receptor B6 antibody
      • Ephb6 antibody
      • EPHB6_HUMAN antibody
      • Ephrin type B receptor 6 precursor antibody
      • Ephrin type-B receptor 6 antibody
      • HEP antibody
      • MGC129910 antibody
      • MGC129911 antibody
      • Tyrosine protein kinase defective receptor EPH 6 antibody
      • Tyrosine-protein kinase-defective receptor EPH-6 antibody
      see all

    Anti-Eph receptor B6 antibody images

    • Eph receptor B6 antibody (ab54656) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human pancreas.


    • Predicted band size : 109 kDa
      Eph receptor B6 antibody (ab54656) at 1ug/lane + PC-12 cell lysate at 25ug/lane.

    References for Anti-Eph receptor B6 antibody (ab54656)

    ab54656 has not yet been referenced specifically in any publications.

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