Anti-EPHA10 antibody - N-terminal (ab75955)

Overview

  • Product name
    Anti-EPHA10 antibody - N-terminal
    See all EPHA10 primary antibodies
  • Description
    Rabbit polyclonal to EPHA10 - N-terminal
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    KLH conjugated synthetic peptide selected from the N terminal region of human EPHA10.

  • Positive control
    • K562 cell line lysates. Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab75955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notes
    ELISA: 1/1000.
    IHC-P: 1/10 - 1/50.
    WB: 1/50 - 1/100. Detects a band of approximately 97 kDa (Isoform 3)(predicted molecular weight: 110 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Receptor for members of the ephrin-A family. Binds to EFNA3, EFNA4 and EFNA5.
    • Tissue specificity
      Mainly expressed in testis.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 1 protein kinase domain.
      Contains 1 SAM (sterile alpha motif) domain.
    • Domain
      The protein kinase domain is predicted to be catalytically inactive.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Form
      Ephrin receptors, the largest subfamily of receptor tyrosine kinases, and their ephrin ligands are important mediators of cell-cell communication regulating cell attachment, shape, and mobility in neuronal and epithelial cells. There are 3 isoforms produced by alternative splicing. Isoform 2 known as Epha10s, Isoform 3 known as Epha10*.
    • Alternative names
      • EPH receptor A10 antibody
      • EPHA 10 antibody
      • Epha10 antibody
      • EphA10s protein antibody
      • EPHAA_HUMAN antibody
      • Ephrin type A receptor 10 antibody
      • Ephrin type-A receptor 10 antibody
      • FLJ16103 antibody
      • FLJ33655 antibody
      • MGC43817 antibody
      see all

    Images

    • Anti-EPHA10 antibody - N-terminal (ab75955) at 1/60 dilution + K562 cell line lysates at 35 µg

      Predicted band size : 110 kDa
      Observed band size : 97 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 36 kDa,60 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.
    • Immunohistochemical analysis of EPHA10 in formalin-fixed, paraffin-embedded human testis tissue using ab75955 at 1/10 dilution, followed by a peroxidase-conjugated secondary antibody and DAB staining.

    References

    ab75955 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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