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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Concentration information loading...
Purity Protein A purified
Purification notes ab75955 is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes ELISA: 1/1000.
IHC-P: 1/10 - 1/50.
WB: 1/50 - 1/100. Detects a band of approximately 97 kDa (Isoform 3)(predicted molecular weight: 110 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function Receptor for members of the ephrin-A family. Binds to EFNA3, EFNA4 and EFNA5.
Tissue specificity Mainly expressed in testis.
Sequence similarities Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.
Domain The protein kinase domain is predicted to be catalytically inactive.
Cellular localization Secreted and Cell membrane.
Information by UniProt
Form Ephrin receptors, the largest subfamily of receptor tyrosine kinases, and their ephrin ligands are important mediators of cell-cell communication regulating cell attachment, shape, and mobility in neuronal and epithelial cells. There are 3 isoforms produced by alternative splicing. Isoform 2 known as Epha10s, Isoform 3 known as Epha10*.
EPH receptor A10 antibody
EPHA 10 antibody
Anti-EPHA10 antibody - N-terminal images
Western blot - EPHA10 antibody - Aminoterminal end (ab75955)
Anti-EPHA10 antibody - N-terminal (ab75955) at 1/60 dilution + K562 cell line lysates at 35 µg Predicted band size : 110 kDa Observed band size : 97 kDa ( why is the actual band size different from the predicted?) Additional bands at : 36 kDa,60 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EPHA10 antibody - N-terminal (ab75955)
Immunohistochemical analysis of EPHA10 in formalin-fixed, paraffin-embedded human testis tissue using ab75955 at 1/10 dilution, followed by a peroxidase-conjugated secondary antibody and DAB staining.
References for Anti-EPHA10 antibody - N-terminal (ab75955)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"