Overview

  • Product nameAnti-Ephexin-1 antibody
    See all Ephexin-1 primary antibodies
  • Description
    Rabbit polyclonal to Ephexin-1
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 1-133 of Human Ephexin-1.

  • Positive control
    • Human cerebral cortex tissue; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab121274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • FunctionActs as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension.
  • Tissue specificityHighly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung.
  • Sequence similaritiesContains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • DomainThe DH domain and the PH domain are both required to mediate interaction with EPHA4.
  • Post-translational
    modifications
    Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA.
  • Cellular localizationCytoplasm. Membrane. Cell projection > growth cone. Associated with membranes. Localizes to axonal growth cones.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGEF27 antibody
    • Eph receptor-interacting exchange protein antibody
    • Eph-interacting exchange protein antibody
    • EPHEXIN antibody
    • Ephexin-1 antibody
    • Guanine nucleotide exchange factor, neuronal antibody
    • Neuronal guanine nucleotide exchange factor antibody
    • Ngef antibody
    • NGEF_HUMAN antibody
    see all

Anti-Ephexin-1 antibody images

  • ab121274 at 1/100 dilution staining Ephexin-1 in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
  • ab121274 at 4 µg/ml staining Ephexin-1 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

References for Anti-Ephexin-1 antibody (ab121274)

ab121274 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"