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Rabbit polyclonal to Ephexin-1 - C-terminal
Mouse, Rat, Human
Predicted to work with:
Synthetic peptide coupled to a carrier protein, corresponding to C-terminal amino acids of Mouse Ephexin-1 (UniProt: Q8CHT1).
A431, Jurkat and HUVEC cell lysates; Rat PC12 cell lysate; Mouse brain tissue lysate; Mouse C2C12 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 82 kDa.
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension.
Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain. Contains 1 SH3 domain.
The DH domain and the PH domain are both required to mediate interaction with EPHA4.
Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA.
Cytoplasm. Membrane. Cell projection > growth cone. Associated with membranes. Localizes to axonal growth cones.
Information by UniProt
Eph receptor-interacting exchange protein antibody
Eph-interacting exchange protein antibody
Western blot - Anti-Ephexin-1 antibody - C-terminal (ab157593)
All lanes : Anti-Ephexin-1 antibody - C-terminal (ab157593) at 1/1000 dilution Lane 1 : A431 cell lysate Lane 2 : A431 cell lysate, treated with pervanadate (1mM; 30 mins) Predicted band size: 82 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Ephexin-1 antibody - C-terminal (ab157593)
Immunofluorescent analysis of Mouse C2C12 cells, labeling Ephexin-1 with ab157593 at 1/50 dilution. Right image shows cells treated with pervanadate.
has not yet been referenced specifically in any publications.
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