Anti-Ephexin-1 (phospho Y87) antibody (ab157594)

Overview

  • Product name
    Anti-Ephexin-1 (phospho Y87) antibody
    See all Ephexin-1 primary antibodies
  • Description
    Rabbit polyclonal to Ephexin-1 (phospho Y87)
  • Specificity
    The specific band detected in WB is removed by alkaline phosphatase treatment.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken
  • Immunogen

    Synthetic phosphopeptide coupled to a carrier protein, corresponding to residues surrounding tyrosine 87 of Mouse Ephexin-1 (UniProt ID: Q8CHT1).

  • Positive control
    • A431 cell lysate; Mouse C2C12 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab157594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 82 kDa.
ICC/IF 1/200.

Target

  • Function
    Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension.
  • Tissue specificity
    Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung.
  • Sequence similarities
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Domain
    The DH domain and the PH domain are both required to mediate interaction with EPHA4.
  • Post-translational
    modifications
    Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA.
  • Cellular localization
    Cytoplasm. Membrane. Cell projection > growth cone. Associated with membranes. Localizes to axonal growth cones.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARHGEF27 antibody
    • Eph receptor-interacting exchange protein antibody
    • Eph-interacting exchange protein antibody
    • EPHEXIN antibody
    • Ephexin-1 antibody
    • Guanine nucleotide exchange factor, neuronal antibody
    • Neuronal guanine nucleotide exchange factor antibody
    • Ngef antibody
    • NGEF_HUMAN antibody
    see all

Images

  • Immunofluorescent analysis of mouse C2C12 cells labeling Exphexin-1 with ab157593 (C-terminal region) (upper images) or ab157594 (Tyr-87) (lower images) at 1/50 dilution, detected using appropriate secondary antibodies conjugated to Cy3. Cells in the right images were treated with pervanadate.
  • Lanes 1 - 2 : Anti-Ephexin-1 antibody - C-terminal (ab157593) at 1/1000 dilution
    Lanes 3 - 4 : Anti-Ephexin-1 (phospho Y87) antibody (ab157594) at 1/1000 dilution

    Lane 1 : Lysate from A431 cells
    Lane 2 : Lysate from A431 cells treated with pervanadate (1 mM) for 30 min.
    Lane 3 : Lysate from A431 cells
    Lane 4 : Lysate from A431 cells treated with pervanadate (1 mM) for 30 min.


    Predicted band size : 82 kDa

References

ab157594 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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