Overview

  • Product nameAnti-Ephrin A1 antibody
    See all Ephrin A1 primary antibodies
  • Description
    Rabbit polyclonal to Ephrin A1
  • Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human Ephrin A1.

  • Positive control
    • HeLa cells, extracts from 293 cells and Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab65072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).

Target

  • FunctionPlays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cones.
  • Tissue specificityBrain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level).
  • Sequence similaritiesBelongs to the ephrin family.
  • Post-translational
    modifications
    Undergo proteolysis by a metalloprotease to give rise to a soluble monomeric form.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B61 antibody
    • ECKLG antibody
    • EFL 1 antibody
    • EFL1 antibody
    • EFNA 1 antibody
    • Efna1 antibody
    • EFNA1_HUMAN antibody
    • EPH related receptor tyrosine kinase ligand 1 antibody
    • EPH-related receptor tyrosine kinase ligand 1 antibody
    • Ephrin-A1 antibody
    • Ephrin-A1, secreted form antibody
    • EphrinA1 antibody
    • EPLG 1 antibody
    • EPLG1 antibody
    • Immediate early response protein B61 antibody
    • LERK 1 antibody
    • LERK-1 antibody
    • LERK1 antibody
    • Ligand of eph related kinase 1 antibody
    • OTTHUMP00000033242 antibody
    • OTTHUMP00000033271 antibody
    • secreted form antibody
    • TNF alpha-induced protein 4 antibody
    • TNFAIP 4 antibody
    • TNFAIP4 antibody
    • Tumor necrosis factor alpha induced protein 4 antibody
    • Tumor necrosis factor alpha-induced protein 4 antibody
    see all

Anti-Ephrin A1 antibody images

  • All lanes : Anti-Ephrin A1 antibody (ab65072) at 1/500 dilution

    Lane 1 : extracts from 293 cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from Jurkat cells with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : >117 kDa. We are unsure as to the identity of these extra bands.
  • ab65072 at 1/500 dilution staining HeLa cells without (left) and with (right) immunizing peptide.
  • All lanes : Anti-Ephrin A1 antibody (ab65072) at 1 µg/ml

    Lane 1 : Recombinant Human Ephrin A1 protein (ab112282) at 0.1 µg
    Lane 2 : Recombinant Human Ephrin A1 protein (ab112282) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 150 seconds

References for Anti-Ephrin A1 antibody (ab65072)

ab65072 has not yet been referenced specifically in any publications.

Product Wall

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The immunogen sequence for ab65072 has no significant homology with the sequence you provided below.

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