• Product name
  • Description
    Rabbit polyclonal to Ephrin A1
  • Tested applications
    Suitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human Ephrin A1.

  • Positive control
    • HeLa cells, extracts from 293 cells and Jurkat cells.



Our Abpromise guarantee covers the use of ab65072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).


  • Function
    Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cones.
  • Tissue specificity
    Brain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level).
  • Sequence similarities
    Belongs to the ephrin family.
  • Post-translational
    Undergo proteolysis by a metalloprotease to give rise to a soluble monomeric form.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B61 antibody
    • ECKLG antibody
    • EFL 1 antibody
    • EFL1 antibody
    • EFNA 1 antibody
    • Efna1 antibody
    • EFNA1_HUMAN antibody
    • EPH related receptor tyrosine kinase ligand 1 antibody
    • EPH-related receptor tyrosine kinase ligand 1 antibody
    • Ephrin-A1 antibody
    • Ephrin-A1, secreted form antibody
    • EphrinA1 antibody
    • EPLG 1 antibody
    • EPLG1 antibody
    • Immediate early response protein B61 antibody
    • LERK 1 antibody
    • LERK-1 antibody
    • LERK1 antibody
    • Ligand of eph related kinase 1 antibody
    • OTTHUMP00000033242 antibody
    • OTTHUMP00000033271 antibody
    • secreted form antibody
    • TNF alpha-induced protein 4 antibody
    • TNFAIP 4 antibody
    • TNFAIP4 antibody
    • Tumor necrosis factor alpha induced protein 4 antibody
    • Tumor necrosis factor alpha-induced protein 4 antibody
    see all


  • All lanes : Anti-Ephrin A1 antibody (ab65072) at 1/500 dilution

    Lane 1 : extracts from 293 cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from Jurkat cells with immunizing peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : >117 kDa. We are unsure as to the identity of these extra bands.
  • ab65072 at 1/500 dilution staining HeLa cells without (left) and with (right) immunizing peptide.
  • All lanes : Anti-Ephrin A1 antibody (ab65072) at 1 µg/ml

    Lane 1 : Recombinant Human Ephrin A1 protein (ab112282) at 0.1 µg
    Lane 2 : Recombinant Human Ephrin A1 protein (ab112282) at 0.01 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 150 seconds


ab65072 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting Abcam.

The immunogen sequence for ab65072 has no significant homology with the sequence you provided below.

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