Recombinant
RabMAb

Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)

Overview

  • Product name
    Anti-Ephrin A1 antibody [EPR1785(2)]
    See all Ephrin A1 primary antibodies
  • Description
    Rabbit monoclonal [EPR1785(2)] to Ephrin A1
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide from the N terminal region of Human Ephrin A1.

  • Positive control
    • HUVEC (treated with TNF alpha), HUVEC (untreated) and MCF7 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cones.
    • Tissue specificity
      Brain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level).
    • Sequence similarities
      Belongs to the ephrin family.
    • Post-translational
      modifications
      Undergo proteolysis by a metalloprotease to give rise to a soluble monomeric form.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • B61 antibody
      • ECKLG antibody
      • EFL 1 antibody
      • EFL1 antibody
      • EFNA 1 antibody
      • Efna1 antibody
      • EFNA1_HUMAN antibody
      • EPH related receptor tyrosine kinase ligand 1 antibody
      • EPH-related receptor tyrosine kinase ligand 1 antibody
      • Ephrin-A1 antibody
      • Ephrin-A1, secreted form antibody
      • EphrinA1 antibody
      • EPLG 1 antibody
      • EPLG1 antibody
      • Immediate early response protein B61 antibody
      • LERK 1 antibody
      • LERK-1 antibody
      • LERK1 antibody
      • Ligand of eph related kinase 1 antibody
      • OTTHUMP00000033242 antibody
      • OTTHUMP00000033271 antibody
      • secreted form antibody
      • TNF alpha-induced protein 4 antibody
      • TNFAIP 4 antibody
      • TNFAIP4 antibody
      • Tumor necrosis factor alpha induced protein 4 antibody
      • Tumor necrosis factor alpha-induced protein 4 antibody
      see all

    Images

    • All lanes : Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911) at 1/1000 dilution

      Lane 1 : HUVEC cell lysate, treated with TNF alpha
      Lane 2 : HUVEC cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 24 kDa
      Observed band size : 24 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Wang Y  et al. HIC1 and miR-23~27~24 clusters form a double-negative feedback loop in breast cancer. Cell Death Differ 24:421-432 (2017). Read more (PubMed: 28009350) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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