Overview

  • Product name
  • Description
    Rabbit polyclonal to Ephrin A2
  • Specificity
    ab72661 detects endogenous levels of fragment of activated Ephrin A2 resulting from cleavage adjacent to Asn188.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from human Ephrin A2

  • Positive control
    • Extracts from 293 cells treated with etoposide (25uM, 1hour).

Properties

Applications

Our Abpromise guarantee covers the use of ab72661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 24 kDa).
ELISA 1/20000.

Target

Images

  • All lanes : Anti-Ephrin A2 antibody (ab72661) at 1/500 dilution

    Lane 1 : Extracts from 293 cells, treated with etoposide (25uM, 1hour)
    Lane 2 : Extracts from 293 cells, treated with etoposide (25uM, 1hour) with Cl-peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 24 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.

References

ab72661 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (many normal and tumor cell lines)
Specification
many normal and tumor cell lines
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Jul 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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