Overview

  • Product nameAnti-Ephrin A3 antibody
    See all Ephrin A3 primary antibodies
  • Description
    Rabbit polyclonal to Ephrin A3
  • Specificityab64814 detects endogenous levels of total Ephrin A3 protein.
  • Tested applicationsSuitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthetic peptide derived from the C-terminal of human Ephrin A3.

  • Positive control
    • Extracts from HepG2 and Jurkat cells. IF/ICC: HeLa cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab64814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 26 kDa).
ELISA 1/10000.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 mg/ml.

Target

  • Tissue specificityExpressed in brain, skeletal muscle, spleen, thymus, prostate, testis, ovary, small intestine, and peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the ephrin family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EFL 2 antibody
    • EFL-2 antibody
    • EFL2 antibody
    • EFN A3 antibody
    • EFNA 3 antibody
    • Efna3 antibody
    • EFNA3_HUMAN antibody
    • EHK1 L antibody
    • EHK1 ligand antibody
    • EHK1-L antibody
    • EHK1L antibody
    • EPH related receptor tyrosine kinase ligand 3 antibody
    • EPH-related receptor tyrosine kinase ligand 3 antibody
    • Ephrin-A3 antibody
    • EphrinA3 antibody
    • EPLG 3 antibody
    • EPLG3 antibody
    • LERK 3 antibody
    • LERK-3 antibody
    • LERK3 antibody
    • Ligand of eph related kinase 3 antibody
    see all

Anti-Ephrin A3 antibody images

  • ICC/IF image of ab64814 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64814, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Ephrin A3 antibody (ab64814) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from Jurkat cells
    Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 26 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-Ephrin A3 antibody (ab64814)

ab64814 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted May 29 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Jurkat cell line)
Specification Jurkat cell line
Permeabilization No
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Verified customer

Submitted Jul 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"