Overview

  • Product nameAnti-Ephrin A5 antibody
    See all Ephrin A5 primary antibodies
  • Description
    Rabbit polyclonal to Ephrin A5
  • Specificityab70114 detects endogenous levels of total Ephrin A5 protein.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human Ephrin A5.

  • Positive control
    • A549 cells, HeLa cell extracts and human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab70114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 22071104ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration. PubMed: 21628570
WB 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 26 kDa).
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionMay function actively to stimulate axon fasciculation. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase.
  • Sequence similaritiesBelongs to the ephrin family.
  • Cellular localizationCell membrane. Membrane > caveola. Compartmentalized in discrete caveolae-like membrane microdomains.
  • Information by UniProt
  • Database links
  • Alternative names
    • EPLG7 antibody
    • AF1 antibody
    • AL 1 antibody
    • AL-1 antibody
    • EFL5 antibody
    • Efna5 antibody
    • EFNA5_HUMAN antibody
    • EPH-related receptor tyrosine kinase ligand 7 antibody
    • Ephrin A5 antibody
    • Ephrin-A5 antibody
    • EPLG7 antibody
    • GLC1M antibody
    • LERK-7 antibody
    • LERK7 antibody
    • RAGS antibody
    see all

Anti-Ephrin A5 antibody images

  • ab70114 staining anti-Ephrin A5 in wild-type and knockout mouse inferior colliculus/cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation with citric acid. Samples were incubated with primary antibody (1/200 in 1 % TBS/BSA buffer) for 2 hours at 20°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab70114 at 1/500 dilution staining Ephrin A5 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab70114 at 1/50 dilution staining Ephrin A5 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide
  • All lanes : Anti-Ephrin A5 antibody (ab70114) at 1/500 dilution

    Lane 1 : HeLa cell extracts
    Lane 2 : HeLa cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 26 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

References for Anti-Ephrin A5 antibody (ab70114)

This product has been referenced in:
  • Son AI  et al. Formation of persistent hyperplastic primary vitreous in ephrin-A5-/- mice. Invest Ophthalmol Vis Sci 55:1594-606 (2014). Read more (PubMed: 24550361) »
  • Brennaman LH  et al. Polysialylated NCAM and EphrinA/EphA Regulate Synaptic Development of GABAergic Interneurons in Prefrontal Cortex. Cereb Cortex : (2012). Mouse . Read more (PubMed: 22275477) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid
Sample Mouse Tissue sections (inferior colliculus/cerebellum)
Specification inferior colliculus/cerebellum
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 04 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brainstem)
Specification Brainstem
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 05 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Cerebellum)
Specification Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Mar 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (flag-tagged mouse ephrinA5 expressed in CHO cells)
Specification flag-tagged mouse ephrinA5 expressed in CHO cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 03 2013

Thank you very much for providing that information. It makes understanding the problems you have been experiencing with the antibody much easier.

I am sorry that you have not obtained the expected results with this antibody. I'd like to ask a...

Read More
Application Flow Cytometry
Sample Human Cell (many normal and tumor cell lines)
Specification many normal and tumor cell lines
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Jul 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"