Synthetic peptide derived from an internal sequence of human Ephrin A5.
Our Abpromise guarantee covers the use of ab70114 in the following tested applications.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 22071104ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21628570|
|WB||1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 26 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/500 - 1/1000.|
ab70114 staining anti-Ephrin A5 in wild-type and knockout mouse inferior colliculus/cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation with citric acid. Samples were incubated with primary antibody (1/200 in 1 % TBS/BSA buffer) for 2 hours at 20°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.