Overview

  • Product nameAnti-Ephrin B antibody
    See all Ephrin B primary antibodies
  • Description
    Rabbit polyclonal to Ephrin B
  • SpecificityThis antibody detects endogenous levels of total Ephrin B protein.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human Ephrin B around the phosphorylation site of Tyrosine 330 (NIYYK).

  • Positive control
    • Extracts from 293 cells treated with EGF

Properties

Applications

Our Abpromise guarantee covers the use of ab55352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/40000.
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa.

Target

  • RelevanceEphrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.
  • Cellular localizationMembrane; single pass type I membrane protein.
  • Database links
  • Alternative names
    • CEK5 ligand antibody
    • EFNB1 antibody
    • ELK L antibody
    • ELK ligand antibody
    • ELKL antibody
    • EPH related receptor tyrosine kinase ligand 2 antibody
    • Ephrin B1 antibody
    • Ephrin B2 antibody
    • LERK2 antibody
    see all

Anti-Ephrin B antibody images

  • All lanes : Anti-Ephrin B antibody (ab55352) at 1/1000 dilution

    Lane 1 : extracts from 293 cells treated with EGF (200 ng/ml for 5 mins) with no immunizing peptide
    Lane 2 : extracts from 293 cells treated with EGF (200 ng/ml for 5 mins) with immunizing peptide

References for Anti-Ephrin B antibody (ab55352)

ab55352 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (Optic tectum)
Specification Optic tectum
Permeabilization Yes - 0.1% TritonX
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

Submitted May 31 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (hippocampus)
Loading amount 5 µg
Specification hippocampus
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 02 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"