The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 37 kDa).
Note: The immunolabeling of the Ephrin B band is blocked by lambda phosphatase treatment.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceEphrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.
Cellular localizationMembrane; single pass type I membrane protein.
EPH related receptor tyrosine kinase ligand 2 antibody
Ephrin B1 antibody
Ephrin B2 antibody
Anti-Ephrin B (phospho Y317) antibody images
Western blot - Ephrin B (phospho Y317) antibody (ab33473)
All lanes : Anti-Ephrin B (phospho Y317) antibody (ab33473) at 1/1000 dilution
Lane 1 : Rat testes lysate treated with this Ephrin B antibody phosphorylated at Tyr317 (control). Lane 2 : Rat testes lysate incubated in lambda phosphatase (1200 unit for 30 min) before being exposed to this Ephrin B antibody phosphorylated at Tyr317.