Anti-Ephrin B (phospho Y317) antibody (ab33473)

Overview

  • Product name
    Anti-Ephrin B (phospho Y317) antibody
    See all Ephrin B primary antibodies
  • Description
    Rabbit polyclonal to Ephrin B (phospho Y317)
  • Specificity
    This antibody is specific to Dak Ephrin B protein phosphorylated at Tyr317.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
  • Immunogen

    Phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr317 of Chicken EphrinB.

  • Positive control
    • Rat testes lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab33473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 37 kDa).
    Note: The immunolabeling of the Ephrin B band is blocked by lambda phosphatase treatment.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.
    • Cellular localization
      Membrane; single pass type I membrane protein.
    • Database links
    • Alternative names
      • CEK5 ligand antibody
      • EFNB1 antibody
      • ELK L antibody
      • ELK ligand antibody
      • ELKL antibody
      • EPH related receptor tyrosine kinase ligand 2 antibody
      • Ephrin B1 antibody
      • Ephrin B2 antibody
      • LERK2 antibody
      see all

    Images

    • All lanes : Anti-Ephrin B (phospho Y317) antibody (ab33473) at 1/1000 dilution

      Lane 1 : Rat testes lysate treated with this Ephrin B antibody phosphorylated at Tyr317 (control).
      Lane 2 : Rat testes lysate incubated in lambda phosphatase (1200 unit for 30 min) before being exposed to this Ephrin B antibody phosphorylated at Tyr317.

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 37 kDa
      Observed band size : 46 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    References

    ab33473 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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