Anti-Ephrin B (phospho Y329) antibody (ab111487)

Overview

  • Product nameAnti-Ephrin B (phospho Y329) antibody
    See all Ephrin B primary antibodies
  • Description
    Rabbit polyclonal to Ephrin B (phospho Y329)
  • Specificityab111487 detects endogenous levels of Ephrin B only when phosphorylated at tyrosine 329 (Human: Tyr329/316; Mouse: Tyr318/319; Rat: Tyr328/-).
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human Ephrin B around the phosphorylation site of tyrosine 329 (P-V-YP-I-V).

  • Positive control
    • Human brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab111487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceEphrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.
  • Cellular localizationMembrane; single pass type I membrane protein.
  • Database links
  • Alternative names
    • CEK5 ligand antibody
    • EFNB1 antibody
    • ELK L antibody
    • ELK ligand antibody
    • ELKL antibody
    • EPH related receptor tyrosine kinase ligand 2 antibody
    • Ephrin B1 antibody
    • Ephrin B2 antibody
    • LERK2 antibody
    see all

Anti-Ephrin B (phospho Y329) antibody images

  • ab111487, at 1/50 dilution, staining Ephrin B in paraffin-embedded Human brain tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

References for Anti-Ephrin B (phospho Y329) antibody (ab111487)

ab111487 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"