Anti-Ephrin B (phospho Y330) antibody (ab51118)

Overview

  • Product nameAnti-Ephrin B (phospho Y330) antibody
    See all Ephrin B primary antibodies
  • Description
    Rabbit polyclonal to Ephrin B (phospho Y330)
  • Specificityab51118 detects endogenous levels of Ephrin B only when phosphorylated at tyrosine 330.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human Ephrin B around the phosphorylation site of tyrosine 330 (N-I-YP-Y-K).

  • Positive control
    • Extracts from 293 cells treated with TNF-a

Properties

Applications

Our Abpromise guarantee covers the use of ab51118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ELISA 1/40000.

Target

  • RelevanceEphrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.
  • Cellular localizationMembrane; single pass type I membrane protein.
  • Database links
  • Alternative names
    • CEK5 ligand antibody
    • EFNB1 antibody
    • ELK L antibody
    • ELK ligand antibody
    • ELKL antibody
    • EPH related receptor tyrosine kinase ligand 2 antibody
    • Ephrin B1 antibody
    • Ephrin B2 antibody
    • LERK2 antibody
    see all

Anti-Ephrin B (phospho Y330) antibody images

  • All lanes : Anti-Ephrin B (phospho Y330) antibody (ab51118)

    Lane 1 : extracts from 293 cells treated with TNF-a (20ng/ml, 30min), pre incubated with immunizing peptide
    Lane 2 : extracts from 293 cells treated with TNF-a (20ng/ml, 30min)


    Predicted band size : 38 kDa

References for Anti-Ephrin B (phospho Y330) antibody (ab51118)

ab51118 has not yet been referenced specifically in any publications.

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