• Product nameAnti-Ephrin B2 antibody
    See all Ephrin B2 primary antibodies
  • Description
    Rabbit polyclonal to Ephrin B2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human Ephrin B2 around the phosphorylation site of tyrosine 316 (P-V-YP-I-V).

  • Positive control
    • HUVEC cells treated with Insulin or EGF.



Our Abpromise guarantee covers the use of ab75868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 37 kDa).


  • FunctionCell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
    (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.
  • Tissue specificityLung and kidney.
  • Sequence similaritiesBelongs to the ephrin family.
    Contains 1 ephrin RBD (ephrin receptor-binding) domain.
  • Post-translational
    Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EFN B2 antibody
    • EFNB 2 antibody
    • Efnb2 antibody
    • EFNB2_HUMAN antibody
    • Eph related receptor tyrosine kinase ligand 5 antibody
    • EPH-related receptor tyrosine kinase ligand 5 antibody
    • ephrin B2 antibody
    • Ephrin-B2 antibody
    • EphrinB2 antibody
    • EPLG 5 antibody
    • EPLG5 antibody
    • Htk L antibody
    • HTK ligand antibody
    • HTK-L antibody
    • HTKL antibody
    • LERK 5 antibody
    • LERK-5 antibody
    • LERK5 antibody
    • Ligand of eph related kinase 5 antibody
    • MGC126226 antibody
    • MGC126227 antibody
    • MGC126228 antibody
    • OTTMUSP00000024973 antibody
    see all

Anti-Ephrin B2 antibody images

  • All lanes : Anti-Ephrin B2 antibody (ab75868) at 1/500 dilution

    Lane 1 : HUVEC cells, untreated with immunising peptide
    Lane 2 : HUVEC cells, treated with EGF
    Lane 3 : HUVEC cells, treated with Insulin

    Predicted band size : 37 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

References for Anti-Ephrin B2 antibody (ab75868)

This product has been referenced in:
  • Ji YJ  et al. EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1. Nat Commun 5:3516 (2014). Read more (PubMed: 24662724) »
  • Moleirinho S  et al. Willin, an upstream component of the hippo signaling pathway, orchestrates mammalian peripheral nerve fibroblasts. PLoS One 8:e60028 (2013). WB ; Mouse . Read more (PubMed: 23593160) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab75868.
Please use the links above to contact us or submit feedback about this product.