The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).
Use at an assay dependent concentration.
FunctionMay play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.
Tissue specificityHighly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.
Sequence similaritiesBelongs to the ephrin family.