• Product nameAnti-Ephrin B3 antibody
    See all Ephrin B3 primary antibodies
  • Description
    Rabbit polyclonal to Ephrin B3
  • SpecificityThis antibody detects endogenous levels of total Ephrin B3 protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthesized peptide derived from human Ephrin B3.

  • Positive control
    • Human brain tissue and extracts from SKOV3 cells



Our Abpromise guarantee covers the use of ab53063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.


  • FunctionMay play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.
  • Tissue specificityHighly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.
  • Sequence similaritiesBelongs to the ephrin family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EFL 6 antibody
    • EFL6 antibody
    • EFNB 3 antibody
    • Efnb3 antibody
    • EFNB3_HUMAN antibody
    • EPH related receptor transmembrane ligand ELK L3 antibody
    • EPH related receptor transmembrane ligand ELKL3 antibody
    • EPH related receptor tyrosine kinase ligand 8 antibody
    • EPH-related receptor transmembrane ligand ELK-L3 antibody
    • EPH-related receptor tyrosine kinase ligand 8 antibody
    • Ephrin B3 antibody
    • Ephrin-B3 antibody
    • EphrinB3 antibody
    • EPLG 8 antibody
    • EPLG8 antibody
    • LERK 8 antibody
    • LERK-8 antibody
    • LERK8 antibody
    see all

Anti-Ephrin B3 antibody images

  • All lanes : Anti-Ephrin B3 antibody (ab53063) at 1/500 dilution

    Lane 1 : Extracts from SKOV3 cells
    Lane 2 : Extracts from SKOV3 cells treated with peptide

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab53063 at a 1:50 dilution.

    Left image un-treated.

    Right image treated with peptide.

  • ICC/IF image of ab53063 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53063, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Ephrin B3 antibody (ab53063)

ab53063 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: tri-sodium citrate buffer
Permeabilization Yes - 0.2% Triton X 100
Specification brain
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde

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Submitted May 21 2015