The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Use at an assay dependent concentration.
Use a concentration of 10 µg/ml.
FunctionMay play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons.
Tissue specificityHighly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.
Sequence similaritiesBelongs to the ephrin family.
ICC/IF image of ab53063 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53063, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Ephrin B3 antibody (ab53063)
has not yet been referenced specifically in any publications.
Publishing research using ab53063? Please let us know so that we can cite the reference in this datasheet.
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