The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml. Tissues require pretreatment with Proteinase K.
FunctionActs on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. Has low phosphatase activity.
Sequence similaritiesBelongs to the AB hydrolase superfamily. Epoxide hydrolase family.
DomainThe N-terminal domain has phosphatase activity. The C-terminal domain has epoxide hydrolase activity.
Post-translational modificationsThe N-terminus is blocked. The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation.