Overview

  • Product name
  • Description
    Mouse polyclonal to EPHX2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length EPHX2 protein (Human)

  • Positive control
    • EPHX2 transfected 293T cell lysate and human kidney tissue lysate

Applications

Our Abpromise guarantee covers the use of ab67788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 63 kDa.

Target

  • Function
    Acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. Has low phosphatase activity.
  • Sequence similarities
    Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.
  • Domain
    The N-terminal domain has phosphatase activity. The C-terminal domain has epoxide hydrolase activity.
  • Post-translational
    modifications
    The N-terminus is blocked.
    The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation.
  • Cellular localization
    Cytoplasm. Peroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bifunctional epoxide hydrolase 2 antibody
    • CEH antibody
    • Cytosolic epoxide hydrolase antibody
    • EPHX2 antibody
    • Epoxide hydratase antibody
    • Epoxide hydrolase 2 antibody
    • Epoxide hydrolase 2 cytoplasmic antibody
    • epoxide hydrolase 2, cytosolic antibody
    • Epoxide hydrolase soluble antibody
    • HYES_HUMAN antibody
    • SEH antibody
    • Soluble epoxide hydrolase antibody
    see all

Anti-EPHX2 antibody images

  • Anti-EPHX2 antibody (ab67788) at 1/500 dilution + human kidney tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 63 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-EPHX2 antibody (ab67788) at 1/500 dilution

    Lane 1 : EPHX2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 63 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

References for Anti-EPHX2 antibody (ab67788)

ab67788 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (Recombinant protein)
Loading amount
0.02 µg
Specification
Recombinant protein
Gel Running Conditions
Reduced Denaturing (5-20% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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