The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMay have a role in bone formation and also in establishing the ordered structure of cartilage through matrix organization.
Tissue specificityCartilage, ligament, and placenta.
Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily. Contains 6 LRR (leucine-rich) repeats. Contains 1 LRRNT domain.
Post-translational modificationsThe O-linked polysaccharides on Thr-60 and Ser-96 are probably the mucin type linked to GalNAc. There is one glycosaminoglycan chain, known to be dermatan sulfate, and it is probably the O-glycosylation at Ser-64.
Cellular localizationSecreted > extracellular space > extracellular matrix.