EpiSeeker ChIP Kit - Histone H4 (acetyl) (ab117149)

Overview

  • Product nameEpiSeeker ChIP Kit - Histone H4 (acetyl)See all Histone H4 acetylation kits ...
  • Sample type
    Adherent cells, Suspension cells
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 acetylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. Reversible acetylation of nucleosomal histone H4 is believed to correlate with potential transcriptionally activity of eukaryotic chromatin domains.

    EpiSeeker ChIP Kit - Histone H4 (acetyl) (ab117149) enables the user to investigate in vivo H4 (acetyl)-DNA interactions within 5 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.

    If you are using tissue as starting material, we recommend the use of EpiSeeker ChIP Kit - Histone H4 (acetyl) - Tissue (ab117151)

  • Notes

    Store Protease Inhibitor Cocktail, Normal Mouse IgG, Anti-Acetyl-Histone H4, Proteinase K and 8-Well Assay Strips at 4°C.

    Store all other components at room temperature.

    The kit is stable for up to 6 months from the shipment date, when stored properly.

  • Tested applicationsChIP more details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 24 tests 48 tests
    8-Well Assay Strips (with Frame) 3 units 6 units
    8-Well Strip Caps 3 units 6 units
    Anti-Acetyl-Histone H4 (1 mg/ml) 1 x 25µl 1 x 50µl
    ECA1 (Wash Buffer) 1 x 28ml 2 x 28ml
    ECA2 (Antibody Buffer) 1 x 15ml 1 x 30ml
    ECA3A (Pre-Lysis Buffer) 1 x 4ml 1 x 8ml
    ECA3B (Lysis Buffer) 1 x 4ml 1 x 6ml
    ECA4 (ChIP Dilution Buffer) 1 x 4ml 1 x 6ml
    ECA5 (DNA Release Buffer) 1 x 1ml 2 x 2ml
    ECA6 (Reverse Buffer) 1 x 1ml 2 x 2ml
    ECA7 (Binding Buffer) 1 x 5ml 1 x 8ml
    ECA8 (Elution Buffer) 1 x 0.6ml 1 x 1.2ml
    F-Collection Tube 30 units 50 units
    F-Spin Column 30 units 50 units
    Normal Mouse IgG (1 mg/ml) 1 x 10µl 1 x 20µl
    Protease Inhibitor Cocktail (100X) 1 x 25µl 1 x 40µl
    Proteinase K (10 mg/ml) 1 x 25µl 1 x 50µl
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab117149 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ChIP Use at an assay dependent concentration.

    EpiSeeker ChIP Kit - Histone H4 (acetyl) images

    • Chromatin was prepared from HeLa cells according to the EpiSeeker ChIP Kit protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 100ml of chromatin (diluted at a 1:1 ratio with ECA4) and 2mg of ab117149 (blue). 2µg of normal Mouse IgG antibody was added as the negative control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

    Protocols

    References for EpiSeeker ChIP Kit - Histone H4 (acetyl) (ab117149)

    ab117149 has not yet been referenced specifically in any publications.

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