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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 acetylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. Reversible acetylation of nucleosomal histone H4 is believed to correlate with potential transcriptionally activity of eukaryotic chromatin domains.
EpiSeeker ChIP Kit - Histone H4 (acetyl) (ab117149) enables the user to investigate in vivo H4 (acetyl)-DNA interactions within 5 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.
If you are using tissue as starting material, we recommend the use of EpiSeeker ChIP Kit - Histone H4 (acetyl) - Tissue (ab117151)
Store Protease Inhibitor Cocktail, Normal Mouse IgG, Anti-Acetyl-Histone H4, Proteinase K and 8-Well Assay Strips at 4°C.
Store all other components at room temperature.
The kit is stable for up to 6 months from the shipment date, when stored properly.
|Components||24 tests||48 tests|
|8-Well Assay Strips (with Frame)||3 units||6 units|
|8-Well Strip Caps||3 units||6 units|
|Anti-Acetyl-Histone H4 (1 mg/ml)||1 x 25µl||1 x 50µl|
|ECA1 (Wash Buffer)||1 x 28ml||2 x 28ml|
|ECA2 (Antibody Buffer)||1 x 15ml||1 x 30ml|
|ECA3A (Pre-Lysis Buffer)||1 x 4ml||1 x 8ml|
|ECA3B (Lysis Buffer)||1 x 4ml||1 x 6ml|
|ECA4 (ChIP Dilution Buffer)||1 x 4ml||1 x 6ml|
|ECA5 (DNA Release Buffer)||1 x 1ml||2 x 2ml|
|ECA6 (Reverse Buffer)||1 x 1ml||2 x 2ml|
|ECA7 (Binding Buffer)||1 x 5ml||1 x 8ml|
|ECA8 (Elution Buffer)||1 x 0.6ml||1 x 1.2ml|
|F-Collection Tube||30 units||50 units|
|F-Spin Column||30 units||50 units|
|Normal Mouse IgG (1 mg/ml)||1 x 10µl||1 x 20µl|
|Protease Inhibitor Cocktail (100X)||1 x 25µl||1 x 40µl|
|Proteinase K (10 mg/ml)||1 x 25µl||1 x 50µl|
Our Abpromise guarantee covers the use of ab117149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
ab117149 has not yet been referenced specifically in any publications.
Publishing research using ab117149 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"