EpiSeeker Fast DNA modification Kit (ab117127)

Overview

  • Product nameEpiSeeker Fast DNA modification KitSee all DNA modification kits ...
  • Tests
    1 x 50 test
  • Assay time
    0h 30m
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Plants, all Mammals
  • Product overview

    DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.

    Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.

    EpiSeeker Fast DNA modification Kit (ab117127) allows DNA denaturation and bisulfite conversion to be processed at the same time so the complete procedure can be performed in only 30 minutes. Furthermore, it prevents more than 90% of DNA loss, completely converting unmethylated cytosine into uracil.

    Traditional methods involve a separate denaturation step followed by a subsequent sodium bisulfite DNA conversion step - but with ab117127, DNA denaturation status is concurrently sustained throughout the entire bisulfite DNA conversion process. ab117127 is suitable for MS-PCR, real time MS-PCR, bisulfite sequencing, pyrosequencing and methylation microarray.

    Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.

  • Notes

    Store all components at room temperature (15-22°C) away from light.

    The kit can be stable for up to 6 months from the shipment date, when stored properly.

  • Tested applicationsFunctional Studies more details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab117127 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration. Minimum DNA amount: 200pg-1µg/ reaction. Optimal DNA amount: 50-200ng/ reaction

    EpiSeeker Fast DNA modification Kit images

    • Complete Cytosine Conversion: 200 ng of genomic DNA isolated from 3 cancer cell lines was treated with ab117127. Next, the unconverted and converted DNA in each treated sample were determined using unconverted DNA-specific and converted DNA-specific primers (ß-actin, 110 bps), respectively. A: real time PCR; B: end-point PCR. The ab117127 treated DNA was completely converted, and no unconverted DNA in the treated samples was determined after 45 cycles.
    • Effective DNA Protection: Fully methylated human genomic DNA at various amounts (0.2 ng-200 ng) were converted using the ab117127. 1 µl of 20 µl eluate was used for real time qPCR and a pair of primers was used to amplify converted DNA. As little as 0.2 ng DNA is sufficient for bisulfite conversion using the ab117127. A: real time PCR; B: starting DNA amount-CT value curve.

    Protocols

    References for EpiSeeker Fast DNA modification Kit (ab117127)

    ab117127 has not yet been referenced specifically in any publications.

    Product Wall

    After bisulfite the DNA will be in ssDNA form and can not be directly used for ligation with illumina's adaptor. We recommend to use ab117127 (enhancer program) to bisulfite modifying DNA after ligation with Illumina's adaptor as indicated by Illumina ...

    Read More

    Thank you for contacting us.

    I am happy to let you know that the EpiSeeker Fast DNA modification Kitab117127is also suitable for bisulfite sequencing and pyrosequencing. Here are several papers related to use of this kit for bisulfite sequen...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"