EpiSeeker Histone H3 (phospho S10) Assay Kit (Colorimetric) (ab115127)

Overview

  • Product nameEpiSeeker Histone H3 (phospho S10) Assay Kit (Colorimetric)See all Histone H3 (S10 Phosphorylation) kits ...
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Sensitivity
    2 ng/well
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Phosphorylation of histone H3 at serine 10 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth. Histone H3 (phospho S10) is regulated by the cell cycle and has been used as a mitotic marker. In vitro studies have shown that H3 (phospho S10) is coupled to acetylation at the nearly K14 residue and that it is negatively impacted by H3 (methyl K9).

    EpiSeeker Histone H3 (phospho S10) Assay Kit (Colorimetric) (ab115127) allows the user to measure global histone H3 (phospho S10) quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it suitable for specifically measuring global histone H3 phophorylation at Ser10 using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.

  • Tested applicationsFunctional Studies more details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab115127 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for EpiSeeker Histone H3 (phospho S10) Assay Kit (Colorimetric) (ab115127)

    ab115127 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab115127.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"