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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
JONATHAN MILNER, CEO
Phosphorylation of histone H3 at serine 10 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth. Histone H3 (phospho S10) is regulated by the cell cycle and has been used as a mitotic marker. In vitro studies have shown that H3 (phospho S10) is coupled to acetylation at the nearly K14 residue and that it is negatively impacted by H3 (methyl K9).
EpiSeeker Histone H3 (phospho S10) Assay Kit (Colorimetric) (ab115127) allows the user to measure global histone H3 (phospho S10) quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it suitable for specifically measuring global histone H3 phophorylation at Ser10 using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
|Components||48 tests||96 tests|
|8-Well Sample Strips (with frame)||4 units||9 units|
|8-Well Standard Control Strips||3 units||2 units|
|ESC1 (10X Wash Buffer)||1 x 10ml||1 x 20ml|
|ESC2 (Antibody Buffer)||1 x 6ml||1 x 12ml|
|ESC3 (Detection Antibody, 1 mg/ml)||1 x 5µl||1 x 10µl|
|ESC4 (Color Developer)||1 x 5ml||1 x 10ml|
|ESC5 (Stop Solution)||1 x 3ml||1 x 6ml|
|Standard Control (100 µg/ml)||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab115127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab115127 has not yet been referenced specifically in any publications.
Publishing research using ab115127 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"