EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)
- Product nameEpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric)See all KDM1 / LSD1 kits ...
- Assay time3h 00m
- Species reactivityReacts with: Mouse, Human, Plants
Predicted to work with: all Mammals, Other
- Product overview
KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.
EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459) is designed for the easy and fast colorimetric measurement of KDM1/LSD1 activity or inhibition. The antibody-based, immunospecific method directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.
Store EKD3, EKD4, and EKD6 at –20°C away from light.
Store EKD1, EKD5, EKD7, EKD8, and 8-Well Assay Strips at 4°C away from light.
Store remaining components (EKD2, EKD9, and Adhesive Covering Film) at room temperature away from light.
- Tested applicationsFunctional Studies more details
- Storage instructionsPlease refer to protocols.
Components 48 tests 96 tests 8-Well Assay Strips (with Frame) 6 units 12 units Adhesive Covering Film 1 unit 1 unit EKD1 (10X Wash Buffer) 1 x 14ml 1 x 28ml EKD2 (LSD1 Assay Buffer) 1 x 4ml 1 x 8ml EKD3 (LSD1 Substrate, 50 µg/ml) 1 x 60µl 1 x 120µl EKD4 (LSD1 Assay Standard, 50 µg/ml) 1 x 10µl 1 x 20µl EKD5 (Capture Antibody, 1000 µg/ml) 1 x 5µl 1 x 10µl EKD6 (Detection Antibody, 400 µg/ml) 1 x 6µl 1 x 12µl EKD7 (LSD1 Inhibitor Tranylcypromine, 1 mM) 1 x 20µl 1 x 40µl EKD8 (Developer Solution) 1 x 5ml 1 x 10ml EKD9 (Stop Solution) 1 x 5ml 1 x 10ml
- Research Areas
- Amine oxidase (flavin containing) domain 2AOF2BHC110
- BRAF35-HDAC complex protein BHC110FAD-binding protein BRAF35-HDAC complex, 110 kDa subunitFlavin-containing amine oxidase domain-containing protein 2KDM1KIAA0601LSD1Lysine (K) specific demethylase 1Lysine (K) specific demethylase 1ALysine-specific histone demethylase 1Lysine-specific histone demethylase 1A
Our Abpromise guarantee covers the use of ab113459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) images
Demonstration of high sensitivity of KDM1 / LSD1 activity assay achieved by using recombinant KDM1 / LSD1 with ab113459.
Demonstration of inhibitory effects of an KDM1/LSD1 inhibitor detected by ab113459. [LSD1 concentration: 200 ng/well]
References for EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)
ab113459 has not yet been referenced specifically in any publications.