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EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)

Q&A (2)

Overview

  • Product nameEpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric)See all KDM1 / LSD1 kits ...
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human, Plants
    Predicted to work with: all Mammals, Other
  • Product overview

    KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.

    EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459) is designed for the easy and fast colorimetric measurement of KDM1/LSD1 activity or inhibition. The antibody-based, immunospecific method directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

  • Notes

    Store EKD3, EKD4, and EKD6 at –20°C away from light.

    Store EKD1, EKD5, EKD7, EKD8, and 8-Well Assay Strips at 4°C away from light.

    Store remaining components (EKD2, EKD9, and Adhesive Covering Film) at room temperature away from light.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceHistone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2)H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor(ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.
    • Alternative names
        Amine oxidase (flavin containing) domain 2AOF2BHC110
        BRAF35-HDAC complex protein BHC110FAD-binding protein BRAF35-HDAC complex, 110 kDa subunitFlavin-containing amine oxidase domain-containing protein 2KDM1KIAA0601LSD1Lysine (K) specific demethylase 1Lysine (K) specific demethylase 1ALysine-specific histone demethylase 1Lysine-specific histone demethylase 1A
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab113459 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Functional Studies FuncS

      EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) images

      • Functional Studies - EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)
        Functional Studies - EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)
        Demonstration of high sensitivity of KDM1 / LSD1 activity assay achieved by using recombinant KDM1 / LSD1 with ab113459.
      • Functional Studies - EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)
        Functional Studies - EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)
        Demonstration of inhibitory effects of an KDM1/LSD1 inhibitor detected by ab113459. [LSD1 concentration: 200 ng/well]

      Protocols

      References for EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Colorimetric) (ab113459)

      ab113459 has not yet been referenced specifically in any publications.

      Publishing research using ab113459 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab113459 Submit a Question for ab113459

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      Votes: Highest First

      Inquiry: I'm looking for a demethylase assay kit for testing of immunocomplexes's activity (containing LSD1) on a possible native substrate (ex recombinant histone rather than peptide). Is your kit 'ab 113472' suitable for this? What is the wave of exc an...

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      Thank you for contacting us.
      The kit ab113472 is designed to detect DNA demethylase activity and will detect the this activity using a unique methylated DNA substrate. The detection is then colorimetric and can be measured by optical density measure ...

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      I am working for Dana Farber Cancer Institute, Boston, MA.Our labis looking for a ELISA kit which can measure the activity of LSD1.For this, we are trying to understand the difference between all the 4 ELISA kits for LSD1(named below) ABcam is providing. ...

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      Thank you for contacting Abcam. For ab113459 and ab113460, both kits directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format. The main difference between the 2 are that ab113459, has a color...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"