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KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.
EpiSeeker KDM1/LSD1 Activity Quantification Assay Kit (Fluorometric)
(ab113460) is designed for the easy and fast fluorometric measurement of KDM1/LSD1 activity or inhibition. The antibody-based, immunospecific method directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.
Store EKD3, EKD4, EKD6 and EKD8 at –20°C away from light.
Store EKD1, EKD5, EKD7, EKD9, and the 8-Well Assay Strips at 4°C away from light.
Store all remaining components (EKD2, EKD10, and the Adhesive Covering Film) at room temperature away from light.
All components of the kit are stable for 6 months from the date of shipment, when stored properly.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Adhesive Covering Film||1 unit||1 unit|
|EKD1 (10X Wash Buffer)||1 x 14ml||1 x 28ml|
|EKD10 (Fluoro Diluter)||1 x 4ml||1 x 8ml|
|EKD2 (LSD1 Assay Buffer)||1 x 4ml||1 x 8ml|
|EKD3 (LSD1 Substrate, 50 µg/ml)||1 x 60µl||1 x 120µl|
|EKD4 (LSD1 Assay Standard, 50 µg/ml)||1 x 10µl||1 x 20µl|
|EKD5 (Capture Antibody, 1000 µg/ml)||1 x 5µl||1 x 10µl|
|EKD6 (Detection Antibody, 400 µg/ml)||1 x 6µl||1 x 12µl|
|EKD7 (LSD1 Inhibitor Tranylcypromine, 1 mM)||1 x 20µl||1 x 40µl|
|EKD8 (Fluoro Developer)||1 x 10µl||1 x 20µl|
|EKD9 (Fluoro Enhancer)||1 x 10µl||1 x 20µl|
Our Abpromise guarantee covers the use of ab113460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113460 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"