EpiSeeker KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (ab113461)

Overview

  • Product nameEpiSeeker KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric)See all KDM4/JMJD2 kits ...
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    KDM4/JMJD2 (Lysine-specific demethylase 4) is a family of histone demethyltransferases involved in H3(meK9) specific demethylation. The KDM4/JMJD2 family are JmjC proteins that specifically removes tri-methylation from H3(meK9) and H3(meK36) but has no activity on mono- or di-methylated residues.

    EpiSeeker KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (ab113461) is designed for the easy and fast colorimetric measurement of KDM4/JMJD2 activity or inhibition. The antibody-based, immunospecific method directly detects KDM4/JMJD2-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

  • Notes

    Store EJM3, EJM4 and EJM6 at –20°C away from light.

    Store EJM1, EJM5, EJM7, Co-factor 1, Co-factor 2, Co-factor 3, and the 8-Well Assay Strips at 4°C away from light.

    Store all remaining components (EJM2, EJM8, and the Adhesive Covering Film) at room temperature away from light.

    All components of the kit are stable for 6 months from the date of shipment, when stored properly.

  • Tested applicationsFunctional Studies more details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab113461 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    EpiSeeker KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) images

    • Demonstration of high sensitivity of the KDM4/JMJD2 activity assay achieved by using recombinant KDM4/JMJD2 with ab113461.

    Protocols

    References for EpiSeeker KDM4/JMJD2 Activity Quantification Assay Kit (Colorimetric) (ab113461)

    ab113461 has not yet been referenced specifically in any publications.

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