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Tanzania, united republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint pierre and miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and herzegovina
JONATHAN MILNER, CEO
KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation. KDM5/JARID demethylases are JmjC proteins that remove di- and tri-methylation from H3(meK4) but have no activity on mono-methylated residues.
EpiSeeker KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464) is designed for the easy and fast fluorometric measurement of KDM5/JARID activity or inhibition. The antibody-based, immunospecific method directly detects KDM5/JARID-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.
Store EJA3, EJA4, EJA6 and EJA7 at –20°C away from light.
Store EJA1, EJA5, EJA8, Co-factor 1, Co-factor 2, Co-factor 3, and the 8-Well Assay Strips at 4°C away from light.
Store all remaining components (EJA2, EJA9, and the Adhesive Covering Film) at room temperature away from light.
All components of the kit are stable for 6 months from the date of shipment, when stored properly.
|Components||48 tests||96 tests|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Adhesive Covering Film||1 unit||1 unit|
|Co-factor 1||1 x 30µl||1 x 60µl|
|Co-factor 2||1 x 30µl||1 x 60µl|
|Co-factor 3||1 x 30µl||1 x 60µl|
|EJA1 (10X Wash Buffer)||1 x 14ml||1 x 28ml|
|EJA2 (JARID Assay Buffer)||1 x 4ml||1 x 8ml|
|EJA3 (JARID Substrate, 50 µg/ml)||1 x 60µl||1 x 120µl|
|EJA4 (JARID Assay Standard, 50 µg/ml)||1 x 10µl||1 x 20µl|
|EJA5 (Capture Antibody, 1000 µg/ml)||1 x 5µl||1 x 10µl|
|EJA6 (Detection Antibody, 400 µg/ml)||1 x 6µl||1 x 12µl|
|EJA7 (Fluoro Developer)||1 x 10µl||1 x 20µl|
|EJA8 (Fluoro Enhancer)||1 x 10µl||1 x 20µl|
|EJA9 (Fluoro Dilution)||1 x 4ml||1 x 8ml|
Our Abpromise guarantee covers the use of ab113464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113464 has not yet been referenced specifically in any publications.
Publishing research using ab113464 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"