EpiSeeker KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464)

Overview

  • Product nameEpiSeeker KDM5/JARID Activity Quantification Assay Kit (Fluorometric)See all KDM5/JARID kits ...
  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation. KDM5/JARID demethylases are JmjC proteins that remove di- and tri-methylation from H3(meK4) but have no activity on mono-methylated residues.

     

    EpiSeeker KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464) is designed for the easy and fast fluorometric measurement of KDM5/JARID activity or inhibition. The antibody-based, immunospecific method directly detects KDM5/JARID-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

  • Notes

    Store EJA3, EJA4, EJA6 and EJA7 at –20°C away from light.

    Store EJA1, EJA5, EJA8, Co-factor 1, Co-factor 2, Co-factor 3, and the 8-Well Assay Strips at 4°C away from light.

    Store all remaining components (EJA2, EJA9, and the Adhesive Covering Film) at room temperature away from light.

    All components of the kit are stable for 6 months from the date of shipment, when stored properly.

     

  • Tested applicationsFunctional Studies more details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab113464 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    EpiSeeker KDM5/JARID Activity Quantification Assay Kit (Fluorometric) images

    • Demonstration of high sensitivity of KDM5/JARID activity assay achieved by using A549 nuclear extracts with ab113464

    Protocols

    References for EpiSeeker KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464)

    ab113464 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your interest in ab113455 & ab113464.

    You are correct that both these kits measure demethylase activity. The major difference between these 2 kits is the substrate captured on the well. For ab113455, the substrate is a dimet...

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    Thank you for your call last week and for your patience while I have been in touch with my colleagues in the lab regarding ab113464.

    The substrate is a short peptide, and the detection antibody binds to mono-methylated histone.

    I ho...

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    Thank you for contacting us. Tri-methylated histone H3-K4 is a substrate for JARID enzyme which removes methyl group from substrate, which is then detected by antibodies present in the kit. So the kit antibodies will detect the JARID de-methyla...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"