Anti-epithelial Sodium Channel gamma antibody (ab3468)

Overview

  • Product name
    Anti-epithelial Sodium Channel gamma antibody
    See all epithelial Sodium Channel gamma primary antibodies
  • Description
    Rabbit polyclonal to epithelial Sodium Channel gamma
  • Specificity
    Detects recombinant rat gamma-epithelial sodium channel (gamma ENaC).
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis, Non human primates, Amphibian
  • Immunogen

    Synthetic peptide corresponding to Human epithelial Sodium Channel gamma aa 630-649.
    Sequence:

    LRLERAFSNQLTDTQMLDEL


    (Peptide available as ab4990)

Properties

Applications

Our Abpromise guarantee covers the use of ab3468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000. Can be blocked with epithelial Sodium Channel gamma peptide (ab4990).
IP Use at an assay dependent concentration. This antibody specifically binds to the unglycosylated, ~75 kDa recombinant rat gamma ENaC protein.

Target

  • Function
    Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
  • Involvement in disease
    Defects in SCNN1G are a cause of Liddle syndrome (LIDDS) [MIM:177200]. It is an autosomal dominant disorder characterized by pseudoaldosteronism and hypertension associated with hypokalemic alkalosis. The disease is caused by constitutive activation of the renal epithelial sodium channel.
    Defects in SCNN1G are the cause of bronchiectasis with or without elevated sweat chloride type 3 (BESC3) [MIM:613071]. A debilitating respiratory disease characterized by chronic, abnormal dilatation of the bronchi and other cystic fibrosis-like symptoms in the absence of known causes of bronchiectasis (cystic fibrosis, autoimmune diseases, ciliary dyskinesia, common variable immunodeficiency, foreign body obstruction). Clinical features include sub-normal lung function, sinopulmonary infections, chronic productive cough, excessive sputum production, and elevated sweat chloride in some cases.
  • Sequence similarities
    Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1G subfamily.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues.
    Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.
  • Cellular localization
    Apical cell membrane. Apical membrane of epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amiloride sensitive epithelial sodium channel gamma subunit antibody
    • Amiloride sensitive sodium channel subunit gamma antibody
    • Amiloride-sensitive sodium channel subunit gamma antibody
    • BESC3 antibody
    • ENaC gamma subunit antibody
    • ENaCG antibody
    • ENaCgamma antibody
    • Epithelial Na(+) channel subunit gamma antibody
    • Epithelial Na+ channel subunit gamma antibody
    • Gamma ENaC antibody
    • Gamma NaCH antibody
    • Gamma-ENaC antibody
    • Gamma-NaCH antibody
    • Nonvoltage gated sodium channel 1 subunit gamma antibody
    • Nonvoltage-gated sodium channel 1 subunit gamma antibody
    • PHA 1 antibody
    • PHA1 antibody
    • SCNEG antibody
    • SCNN 1G antibody
    • SCNN1G antibody
    • SCNNG_HUMAN antibody
    • Sodium channel epithelial 1 gamma subunit antibody
    • Sodium channel non voltage gated 1 gamma subunit antibody
    • Sodium channel nonvoltage gated 1 gamma antibody
    see all

Anti-epithelial Sodium Channel gamma antibody images

  • All lanes : Anti-epithelial Sodium Channel gamma antibody (ab3468) at 1/2000 dilution

    Lane 1 : H441 whole cell lysate (treated with dexamethasone)
    Lane 2 : H441 whole cell lysate (treated with dexamethasone) with Immunizing peptide

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP conjugated donkey anti-rabbit
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 90-95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa (possible non-specific binding).

    Exposure time : 15 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab3468 staining epithelial Sodium Channel gamma in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% Donkey serum for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/300 in KPBS-BT2) for 12 hours at 4°C. An undiluted HRP-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-epithelial Sodium Channel gamma antibody (ab3468)

This product has been referenced in:
  • Lucas R  et al. The Lectin-like Domain of TNF Increases ENaC Open Probability through a Novel Site at the Interface between the Second Transmembrane and C-terminal Domains of the a-Subunit. J Biol Chem 291:23440-23451 (2016). Read more (PubMed: 27645999) »
  • Wakui H  et al. Renal tubule angiotensin II type 1 receptor-associated protein promotes natriuresis and inhibits salt-sensitive blood pressure elevation. J Am Heart Assoc 4:e001594 (2015). WB ; Mouse . Read more (PubMed: 25792129) »

See all 13 Publications for this product

Product Wall

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Nein, es handelt sich um kein Missverständnis: Ich wollte mit den Wikipedia Eintragen nur verdeutlichen, dass es bei HEK293 Zellen nicht ganz klar ist, um welche Art von Zellen es sich handelt; bzw. ob...

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Es kann gut sein, dass HEK293 Zellen dieses Protein bereits endogen expremieren. Wir selber haben dies allerdings nicht gemessen.

Es gibt allerdings Hinweise, dass dies tatsächlich der Fall sein könnte: Aus Wikipedia "For ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Specification
Kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: heat with pH 6.4
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
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Abcam user community

Verified customer

Submitted May 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Kidney)
Loading amount
10 µg
Specification
Kidney
Treatment
1X10^-6 M Vp for 30 min
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted May 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (bronchial)
Loading amount
30 µg
Specification
bronchial
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (H441)
Loading amount
50 µg
Specification
H441
Treatment
dexamethasone
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Nov 30 2007

Thank you for your enquiry and I apologize for the confusing information that is provided for ab2906 and ab3468. I will verify whether these Abs work in WBs. Once I have that information, I will correct the datasheets and e-mail a copy to you. ...

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