Epithelial to Mesenchymal Transition (EMT) Marker Panel (ab216833)

Overview

  • Product name
    Epithelial to Mesenchymal Transition (EMT) Marker Panel
  • Product overview

    ab216833 is an epithelial to mesenchymal (EMT) marker sampler panel containing 4 antibodies and 1 anti-rabbit secondary: 10 µl E-cadherin rabbit monoclonal, 10 µl N-cadherin rabbit monoclonal, 10 µg snail + slug, 10 µl vimentin rabbit monoclonal, and 100 µg anti-rabbit (HRP).


    EMT is a reversible process where epithelial cells undergo defined molecular changes to become motile mesenchymal cells. This EMT marker sampler panel is a cost-effective and convenient means of evaluating EMT.


    The antibodies in this panel were selected for their exceptional performance in IHC in human tissues. These antibodies have also been tested in a number other applications and species. Please see the individual datasheets for additional information.

Properties

Images

  • Fluorescent immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab40772. Green-E-Cadherin red-PI

  • IHC image of ab85936 staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85936, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab76011 staining N Cadherin in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA + 1% FBS for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer pH6. Samples were incubated with primary antibody (1/500 in 1% BSA + 1% FBS) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • IHC image of unpurified ab92547 staining Vimentin in human breast adenocarcinoma formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab92547, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab216833 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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