Overview

  • Product name
  • Description
    Rabbit polyclonal to EPLIN
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 473-730 of Human EPLIN (UniProt Q9UHB6).

  • Positive control
    • HeLa and HepG2 whole cell lysates; HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 85 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments.
  • Tissue specificity
    Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine. Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium.
  • Sequence similarities
    Contains 1 LIM zinc-binding domain.
  • Domain
    Contains at least 2 actin-binding domains, one on each side of the LIM domain. Both domains bind actin monomers and filaments. The C-terminal domain binds filaments more efficiently than the N-terminus.
  • Cellular localization
    Cytoplasm. Cell junction > focal adhesion. Cytoplasm > cytoskeleton. This cytoskeletal protein co-localizes with actin stress fibers and focal adhesion plaques.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110021C24Rik antibody
    • Epithelial protein lost in neoplasm antibody
    • Epithelial protein lost in neoplasm beta antibody
    • EPLIN antibody
    • FLJ38853 antibody
    • LIM domain and actin binding 1 antibody
    • LIM domain and actin binding protein 1 antibody
    • LIM domain and actin-binding protein 1 antibody
    • LIMA1 antibody
    • LIMA1_HUMAN antibody
    • MGC131726 antibody
    • SREBP3 antibody
    • Sterol regulatory element binding protein 3 antibody
    see all

Images

  • All lanes : Anti-EPLIN antibody (ab154530) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 85 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling EPLIN with ab154530 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

References

ab154530 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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