Anti-EPLIN antibody (ab50196)

Overview

  • Product nameAnti-EPLIN antibody
    See all EPLIN primary antibodies
  • Description
    Rabbit polyclonal to EPLIN
  • SpecificityThis antibody reacts with EPLIN
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog, Pig
  • Immunogen

    A region within synthetic peptide: GVLAASMEAK ASSQQEKEDK PAETKKLRIA WPPPTELGSS GSALEEGIKM, corresponding to amino acids 502-551 of Human EPLIN

  • Positive control
    • Placenta lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab50196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).Can be blocked with Human EPLIN peptide (ab111676). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/12500.

Target

  • FunctionBinds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments.
  • Tissue specificityHighly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine. Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium.
  • Sequence similaritiesContains 1 LIM zinc-binding domain.
  • DomainContains at least 2 actin-binding domains, one on each side of the LIM domain. Both domains bind actin monomers and filaments. The C-terminal domain binds filaments more efficiently than the N-terminus.
  • Cellular localizationCytoplasm. Cell junction > focal adhesion. Cytoplasm > cytoskeleton. This cytoskeletal protein co-localizes with actin stress fibers and focal adhesion plaques.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110021C24Rik antibody
    • Epithelial protein lost in neoplasm antibody
    • Epithelial protein lost in neoplasm beta antibody
    • EPLIN antibody
    • FLJ38853 antibody
    • LIM domain and actin binding 1 antibody
    • LIM domain and actin binding protein 1 antibody
    • LIM domain and actin-binding protein 1 antibody
    • LIMA1 antibody
    • LIMA1_HUMAN antibody
    • MGC131726 antibody
    • SREBP3 antibody
    • Sterol regulatory element binding protein 3 antibody
    see all

Anti-EPLIN antibody images

  • Anti-EPLIN antibody (ab50196) at 2 µg/ml + Placental lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 85 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References for Anti-EPLIN antibody (ab50196)

ab50196 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email. I have checked the protocol you have kindly sent and would like to make few suggestions that I am sure will help to get staining at right location; - Use 5% BSA blocking solution for 1-1.5 hours. - Try using more dil...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Kidney)
Specification Kidney
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C
Username

晴子 鶴見

Verified customer

Submitted Nov 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"