ab112495 (5µg/ml) shows staining of EPM2AIP1 in paraffin embedded Human Heart following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows regular staining of sarcoplasmic reticulum in the cardiac fibers.
Western blot - Anti-EPM2AIP1 antibody (ab112495)
Lane 1 : Anti-EPM2AIP1 antibody (ab112495) at 1 µg/ml Lane 2 : Anti-EPM2AIP1 antibody (ab112495) at 1 mg/ml Lane 3 : anti-DYKDDDDK Tag antibody at 1/1000 dilution
Lanes 1 & 3 : HEK293 lysate overexpressing Human EPM2AIP1 with DYKDDDDK tag Lane 2 : Mock-transfected HEK293
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa
Primary incubations were for 1 hour.
Detected by chemiluminescence.