TRNQHTLSQPLTDEHby a Cysteine residue linker, corresponding to internal sequence amino acids 565-579 of Human EPM2AIP1 (NP_055620.1).
Our Abpromise guarantee covers the use of ab112495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 70 kDa.|
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab112495 (5µg/ml) shows staining of EPM2AIP1 in paraffin embedded Human Heart following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows regular staining of sarcoplasmic reticulum in the cardiac fibers.
ab112495 has not yet been referenced specifically in any publications.