• Product nameAnti-EPO antibody
    See all EPO primary antibodies
  • Description
    Rabbit polyclonal to EPO
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Non human primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human EPO.

    (Peptide available as ab88211.)

  • Positive control
    • This antibody gave a positive signal in human liver and human kidney tissue lysates.



Our Abpromise guarantee covers the use of ab65394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 21 kDa).


  • RelevanceHuman erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • EP antibody
    • EPO alpha antibody
    • EPO antibody
    • Epoetin antibody
    • Erythropoetin antibody
    • Erythropoietin precursor antibody
    • MVCD2 antibody
    see all

Anti-EPO antibody images

  • All lanes : Anti-EPO antibody (ab65394) at 1 µg/ml (Blocked with 1% Milk)

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 21 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa,60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-EPO antibody (ab65394)

This product has been referenced in:
  • Wang X  et al. A validated model of the pro- and anti-inflammatory cytokine balancing act in articular cartilage lesion formation. Front Bioeng Biotechnol 3:25 (2015). Read more (PubMed: 25806365) »
  • Baker JM  et al. Endurance exercise training promotes medullary hematopoiesis. FASEB J : (2011). WB ; Mouse . Read more (PubMed: 21868472) »

See all 2 Publications for this product

Product Wall

Thank you for your response. Unfortunately, none of these antibodies (EPO) have been specifically tested in IP. You may be interested in our testing discount scheme. The Terms and Conditions of this offer can be found at: www.abcam.com/collaboration...

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Thank you for your enquiry and your interest. There are different alternative names for erythropoietin, please see some listed below: EP antibody EPO alpha antibody EPO antibody Epoetin antibody Ery...

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Thank you for getting back to me. I can understand now the reason for selecting different EPO antibodies as free of charge replacements. This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial...

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Thank you for your response. I can certainly arrange two free of charge replacement vials or credit note for your troubled customer. It is not a problem at all. I understand that the customer has successfully used ab65394 and compared the results w...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with these two products. Could you please provide the Abcam O...

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