Overview

  • Product nameAnti-EPO antibody [Epo1]
    See all EPO primary antibodies
  • Description
    Mouse monoclonal [Epo1] to EPO
  • Tested applicationsSuitable for: ELISA, WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab20473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 21 kDa. Dilution optimised using Chromogenic detection.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [Epo2] to EPO (ab20375).

Target

  • RelevanceHuman erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • EP antibody
    • EPO alpha antibody
    • EPO antibody
    • Epoetin antibody
    • Erythropoetin antibody
    • Erythropoietin precursor antibody
    • MVCD2 antibody
    see all

References for Anti-EPO antibody [Epo1] (ab20473)

This product has been referenced in:
  • Shin SK  et al. Application of a bridging ELISA for detection of anti-erythropoietin binding antibodies and a cell-based bioassay for neutralizing antibodies in human sera. J Pharm Biomed Anal 52:289-93 (2010). ELISA ; Human . Read more (PubMed: 20133098) »

See 1 Publication for this product

Product Wall

Thank you for contacting us and for sharing your results with us.

I have happy to hear that we were able to improve your results for the sELISA using EPO1 and EPO2.

I am sorry to hear that ab30545 is still not performing even with t...

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As far as I understand from customer's email they are having problem with ab30545 and ab20375 in sandwich ELISA. Please note these antibodies has not been tested together in sandwich ELISA so I am sorry we will not be...

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I am sorry to hear that you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I ...

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The antibody ab20473 is of the clone number EpoI, as is ab110656 and should work well in a pair for sandwich ELISA with ab20375. Please do however bear in mind that both antibodie...

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Thank you for contacting us and your interest in our products.

The epitope recognized on erythropoietin by the Anti-EPO antibody [Epo1] (ab20473) has unfortunately not been mapped as yet.This antibody has however been tested in Western blot a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"