Anti-EPO antibody [Epo2] (ab20375)

Overview

  • Product nameAnti-EPO antibody [Epo2]
    See all EPO primary antibodies
  • Description
    Mouse monoclonal [Epo2] to EPO
  • Tested applicationsSuitable for: IHC-P, ELISA, WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab20375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [Epo1] to EPO (ab20473).

Target

  • RelevanceHuman erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • EP antibody
    • EPO alpha antibody
    • EPO antibody
    • Epoetin antibody
    • Erythropoetin antibody
    • Erythropoietin precursor antibody
    • MVCD2 antibody
    see all

Anti-EPO antibody [Epo2] images

  • IHC image of ab20375 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20375, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-EPO antibody [Epo2] (ab20375)

ab20375 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and for sharing your results with us.

I have happy to hear that we were able to improve your results for the sELISA using EPO1 and EPO2.

I am sorry to hear that ab30545 is still not performing even with t...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that these antibodies are not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information...

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Thank you for your email.

As far as I understand from customer's email they are having problem with ab30545 and ab20375 in sandwich ELISA. Please note these antibodies has not been tested together in sandwich ELISA so I am sorry we will not be...

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Thank you for contacting us.

I am sorry to hear that you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I ...

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Thank you for contacting us and your interest in our products.

The antibody ab20473 is of the clone number EpoI, as is ab110656 and should work well in a pair for sandwich ELISA with ab20375. Please do however bear in mind that both antibodie...

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Thank you for your inquiry.

I can confirm that ab20375 is un-conjugated and therefore not linked to an enzyme or a fluorochrome.

I hope this information is helpful and wish you a good start into the new week.

Thank you for your response. Unfortunately, none of these antibodies (EPO) have been specifically tested in IP. You may be interested in our testing discount scheme. The Terms and Conditions of this offer can be found at: www.abcam.com/collaboration...

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Thank you for your enquiry and your interest. There are different alternative names for erythropoietin, please see some listed below: EP antibody EPO alpha antibody EPO antibody Epoetin antibody Ery...

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Thank you for getting back to me. I can understand now the reason for selecting different EPO antibodies as free of charge replacements. This is to let you know that I have confirmed and agreed on placing a new order for your customer - for one vial...

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Thank you for your response. I can certainly arrange two free of charge replacement vials or credit note for your troubled customer. It is not a problem at all. I understand that the customer has successfully used ab65394 and compared the results w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"