Overview

  • Product name
    Anti-EPO Receptor antibody
    See all EPO Receptor primary antibodies
  • Description
    Goat polyclonal to EPO Receptor
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human), expressed in mouse myeloma NSO cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab10653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
ELISA
  • Application notes
    ELISA: Use at a concentration of 0.5 - 1 µg/ml. The detection limit is approximately 0.3 ng/well.

    IHC-P: Use at an assay depependent dilution (PMID 19544471).

    WB: Use at a concentration of 0.1 - 2 µg/ml. Detects a band of approximately 50 kDa. The detection limit is approximately 5 ng/lane under non-reducing and reducing conditions.

    Endotoxin: < 10 ng/mg of antibody determined by the LAL method.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
      Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.
    • Tissue specificity
      Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.
    • Involvement in disease
      Defects in EPOR are the cause of erythrocytosis familial type 1 (ECYT1) [MIM:133100]. ECYT1 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.
    • Sequence similarities
      Belongs to the type I cytokine receptor family. Type 1 subfamily.
      Contains 1 fibronectin type-III domain.
    • Domain
      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.
      Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Post-translational
      modifications
      On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.
      Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.
    • Cellular localization
      Cell membrane and Secreted. Secreted and located to the cell surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • EPO R antibody
      • EPO-R antibody
      • epor antibody
      • EPOR_HUMAN antibody
      • Erythropoietin receptor antibody
      • Erythropoietin receptor precursor antibody
      • MGC138358 antibody
      see all

    References

    This product has been referenced in:

    See all 7 Publications for this product

    Customer reviews and Q&As

    Thank you for contacting us.

    Any fluorophore conjugated secondary antibody can be used as long as it is anti goat.

    The few example are

    ab96931, ab96935, ab96932, ab96916 or ab96917

    I hope this information is helpful ...

    Read More

    Thank you for contacting us.

    These antibodies haven't yet been tested in ICC/IF, this however does not mean these can not be used.

    We can provide 100% Abreview testing discount if the customer is interested in testing any of these an...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Spleen)
    Loading amount
    20 µg
    Specification
    Spleen
    Gel Running Conditions
    Reduced Denaturing (4-20% polyacrylamide Bio-Rad ReadyGel, Tris buffer)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Mr. Michael Saul

    Verified customer

    Submitted Jul 28 2011

    Thank you for your enquiry. I have received confirmation from our laboratory that this product does not contain any gelatin or sodium azide preservative. I hope this confirmation will help clear your concerns about this product. Should you have a...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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