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Synthetic non-phosphopeptide from human EPO Receptor, designed around the phosphorylation site of tyrosine 368 (DTYPLV)
Our Abpromise guarantee covers the use of ab61162 in the following tested applications.
|WB||1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
ab166162 staining EPO Receptor in NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serim for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hour at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.