Anti-EPO Receptor antibody [MM-0031-6G7] (ab56310)


  • Product nameAnti-EPO Receptor antibody [MM-0031-6G7]
    See all EPO Receptor primary antibodies
  • Description
    Mouse monoclonal [MM-0031-6G7] to EPO Receptor
  • SpecificityAb56310 recognizes EPO Receptor using western blot. This antibody has been tested to be able to pick up human EPOR around 60-65 kDa under both reducing and non-reducing conditions. However the immunosignal is much stronger under non-reducing conditions so remove beta-mercaptoethanol or DTT from sample buffer.
  • Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human recombinant EPO Receptor (extracellular domain)



Our Abpromise guarantee covers the use of ab56310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/40. Proteinase k digestion is required, 20 ug/ml proteinase K (in PBS), digestion at room temperature for 15-30 mins.
WB 1/1000 - 1/2000. Use under non reducing condition. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • FunctionReceptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
    Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.
  • Tissue specificityErythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.
  • Involvement in diseaseDefects in EPOR are the cause of erythrocytosis familial type 1 (ECYT1) [MIM:133100]. ECYT1 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 1 fibronectin type-III domain.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Post-translational
    On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.
    Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.
  • Cellular localizationCell membrane and Secreted. Secreted and located to the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • EPO R antibody
    • EPO-R antibody
    • epor antibody
    • EPOR_HUMAN antibody
    • Erythropoietin receptor antibody
    • Erythropoietin receptor precursor antibody
    • MGC138358 antibody
    see all

Anti-EPO Receptor antibody [MM-0031-6G7] images

  • Formalin fixed and Paraffin embedded human placenta tissue section was subjected to IHC staining using ab56310 (Proteinase K pretreatment is required).
  • Overlay histogram showing K562 cells stained with ab56310 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56310, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-EPO Receptor antibody [MM-0031-6G7] (ab56310)

This product has been referenced in:
  • McGraw KL  et al. Erythropoietin receptor signaling is membrane raft dependent. PLoS One 7:e34477 (2012). WB ; Human . Read more (PubMed: 22509308) »
  • Chen AY  et al. Productive Parvovirus B19 Infection of Primary Human Erythroid Progenitor Cells at Hypoxia Is Regulated by STAT5A and MEK Signaling but not HIFa. PLoS Pathog 7:e1002088 (2011). WB, Flow Cyt ; Human . Read more (PubMed: 21698228) »

See all 4 Publications for this product

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According to the datasheet, we have used a human recombinant EPO Receptor (extracellular domain) as the immunogen. As this protein is a single-pass type I membrane protein, the immunogen can be found within range...

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ab54659: 100%ABR-00000

ab56310: 100%ABR-00000

Ablaufdatum: 01. Februar 2013

Es freut mich zu hören, dass Sie unser Angebot die Antikörper ab54659 und ab56310 inKaninchen z...

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Vielen Dank für Ihren Vorschlag.

Die Homologie des Immunogen von ab56310 mit Maus liegt bei 81%.

Das ist eher grenzwertig für einen monoklonalen Antikörper.

Ich stimme Ihnen dennoch zu, dass es wahrscheinlic...

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Thank you for contacting us.

These antibodies haven't yet been tested in ICC/IF, this however does not mean these can not be used.

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