Anti-Epoxide hydrolase antibody [15B2AD9] (ab110307)

Overview

  • Product name
    Anti-Epoxide hydrolase antibody [15B2AD9]
    See all Epoxide hydrolase primary antibodies
  • Description
    Mouse monoclonal [15B2AD9] to Epoxide hydrolase
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, ICC/IF, In-Cell ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human liver mitochondria.

  • Positive control
    • Human HDFn cells Human normal colon FFPE tissue
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    The purity of ab110307 is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • Clonality
    Monoclonal
  • Clone number
    15B2AD9
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.
In-Cell ELISA Use a concentration of 4 µg/ml. (0.4 µg/well).
IP Use at an assay dependent concentration.

Target

  • Function
    Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water.
  • Tissue specificity
    Found in liver.
  • Involvement in disease
    Note=In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls.
    Defects in EPHX1 are a cause of familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
  • Sequence similarities
    Belongs to the peptidase S33 family.
  • Cellular localization
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EPHX 1 antibody
    • EPHX antibody
    • Ephx1 antibody
    • EPOX antibody
    • Epoxide hydratase antibody
    • Epoxide hydrolase 1 antibody
    • Epoxide hydrolase 1 microsomal (xenobiotic) antibody
    • Epoxide hydroxylase 1 antibody
    • Epoxide hydroxylase 1 microsomal (xenobiotic) antibody
    • HYEP_HUMAN antibody
    • HYL1 antibody
    • MEH antibody
    • Microsomal epoxide hydrolase antibody
    see all

Anti-Epoxide hydrolase antibody [15B2AD9] images

  • Overlay histogram showing HepG2 cells stained with ab110307 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110307, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunocytochemistry image of ab110307-stained Human HDFn cells.
    The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab110307 at 4 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue).
    Target protein locates mainly in microsomal/ER.
  • IHC image of Epoxide hydrolase staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110307, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

References for Anti-Epoxide hydrolase antibody [15B2AD9] (ab110307)

ab110307 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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