Anti-Epoxide hydrolase antibody (ab96774)
Key features and details
- Rabbit polyclonal to Epoxide hydrolase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Epoxide hydrolase antibody
See all Epoxide hydrolase primary antibodies -
Description
Rabbit polyclonal to Epoxide hydrolase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig -
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 64 and 262 of human Epoxide hydrolase (NP_000111)
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Positive control
- 293T, H1299 and HeLa cells. SG xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
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IHC-P | (1) |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. -
Tissue specificity
Found in liver. -
Involvement in disease
Note=In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls.
Defects in EPHX1 are a cause of familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption. -
Sequence similarities
Belongs to the peptidase S33 family. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 535293 Cow
- Entrez Gene: 2052 Human
- Entrez Gene: 13849 Mouse
- Entrez Gene: 397639 Pig
- Entrez Gene: 100009104 Rabbit
- Entrez Gene: 25315 Rat
- Omim: 132810 Human
- SwissProt: P07099 Human
see all -
Alternative names
- EPHX 1 antibody
- EPHX antibody
- Ephx1 antibody
see all
Images
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All lanes : Anti-Epoxide hydrolase antibody (ab96774) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
Gel conc 10% (SDS PAGE) -
Immunohistochemical analysis of paraffin-embedded SG xenograft, using ab96774 at 1/100 dilution.
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Immunofluorescence analysis of methanol fixed HeLa, using ab96774 at 1/200 dilution (top image) and costained with Hoescht 33342 (bottom image).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab96774 has been referenced in 1 publication.
- Ortiz C et al. Cytotoxicity and Genotoxicity Assessment of Sandalwood Essential Oil in Human Breast Cell Lines MCF-7 and MCF-10A. Evid Based Complement Alternat Med 2016:3696232 (2016). WB . PubMed: 27293457