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Storage instructions Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
1/100 - 1/500.
1/100 - 1/200.
Function Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water.
Tissue specificity Found in liver.
Involvement in disease Note=In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls. Defects in EPHX1 are a cause of familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
Sequence similarities Belongs to the peptidase S33 family.
Cellular localization Microsome membrane. Endoplasmic reticulum membrane.
Information by UniProt
EPHX 1 antibody
Anti-Epoxide hydrolase antibody images
Western blot - Epoxide hydrolase antibody (ab96774)
All lanes : Anti-Epoxide hydrolase antibody (ab96774) at 1/1000 dilution Lane 1 : H1299 whole cell lysate Lane 2 : HeLa whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size : 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Epoxide hydrolase antibody (ab96774)
Immunohistochemical analysis of paraffin-embedded SG xenograft, using ab96774 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Epoxide hydrolase antibody (ab96774)
Immunofluorescence analysis of methanol fixed HeLa, using ab96774 at 1/200 dilution (top image) and costained with Hoescht 33342 (bottom image).
References for Anti-Epoxide hydrolase antibody (ab96774)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"