Overview

  • Product name
    Anti-Epoxide hydrolase antibody
    See all Epoxide hydrolase primary antibodies
  • Description
    Rabbit polyclonal to Epoxide hydrolase
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 64 and 262 of human Epoxide hydrolase (NP_000111)

  • Positive control
    • 293T, H1299 and HeLa cells. SG xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab96774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 53 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water.
  • Tissue specificity
    Found in liver.
  • Involvement in disease
    Note=In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls.
    Defects in EPHX1 are a cause of familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.
  • Sequence similarities
    Belongs to the peptidase S33 family.
  • Cellular localization
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EPHX 1 antibody
    • EPHX antibody
    • Ephx1 antibody
    • EPOX antibody
    • Epoxide hydratase antibody
    • Epoxide hydrolase 1 antibody
    • Epoxide hydrolase 1 microsomal (xenobiotic) antibody
    • Epoxide hydroxylase 1 antibody
    • Epoxide hydroxylase 1 microsomal (xenobiotic) antibody
    • HYEP_HUMAN antibody
    • HYL1 antibody
    • MEH antibody
    • Microsomal epoxide hydrolase antibody
    see all

Images

  • All lanes : Anti-Epoxide hydrolase antibody (ab96774) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 53 kDa
  • Immunohistochemical analysis of paraffin-embedded SG xenograft, using ab96774 at 1/100 dilution.
  • Immunofluorescence analysis of methanol fixed HeLa, using ab96774 at 1/200 dilution (top image) and costained with Hoescht 33342 (bottom image).

References

This product has been referenced in:
  • Ortiz C  et al. Cytotoxicity and Genotoxicity Assessment of Sandalwood Essential Oil in Human Breast Cell Lines MCF-7 and MCF-10A. Evid Based Complement Alternat Med 2016:3696232 (2016). WB . Read more (PubMed: 27293457) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab96774.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up