Overview

  • Product nameAnti-EPS15 antibody - C-terminal
    See all EPS15 primary antibodies
  • Description
    Rabbit polyclonal to EPS15 - C-terminal
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide selected from the C-terminal region of human EPS15 conjugated to KLH.

  • Positive control
    • HL60 cell line lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab71272 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
ELISA 1/1000.

Target

  • RelevanceThe eps15 protein (EGFR pathway substrate clone 15) is tyrosine phosphorylated by the activation of the EGF and PDGF receptors. It was first identified as an 896 amino acid protein in mouse. Subsequent cloning of the human protein showed 90% homology to the mouse form. Several physiological functions have been proposed for eps15. Phosphorylation by EGF and PDGF receptors indicates that eps15 plays a role in signal transduction, cell proliferation and endocytosis.
  • Cellular localizationCytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits.
  • Database links
  • Alternative names
    • AF 1P antibody
    • AF 1p protein antibody
    • AF1P antibody
    • ALL1 fused gene from chromosome 1 antibody
    • Epidermal growth factor receptor pathway substrate 15 antibody
    • Epidermal growth factor receptor substrate 15 antibody
    • EPS 15 antibody
    • MLLT 5 antibody
    • MLLT5 antibody
    • Protein Eps 15 antibody
    • Protein Eps15 antibody
    see all

Anti-EPS15 antibody - C-terminal images

  • Anti-EPS15 antibody - C-terminal (ab71272) at 1/100 dilution + HL60 cell line lysate at 35 µg

    Predicted band size : 99 kDa
    Observed band size : 99 kDa
    Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.

References for Anti-EPS15 antibody - C-terminal (ab71272)

ab71272 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"