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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Concentration information loading...
Purity Protein G purified
Purification notes ab71728 purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 104 kDa (predicted molecular weight: 94 kDa).
Function Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
Sequence similarities Contains 1 EF-hand domain. Contains 3 EH domains.
Post-translational modifications Phosphorylated on tyrosine residues by EGFR.
Cellular localization Cell membrane. Nucleus. Membrane > coated pit. Localized to plasma membrane coated pits.
Information by UniProt
epidermal growth factor receptor pathway substrate 15- ike 1 antibody
Epidermal growth factor receptor substrate 15 like 1 antibody
Anti-EPS15R antibody - Aminoterminal end images
Western blot - EPS15R antibody - Aminoterminal end (ab71728)
Anti-EPS15R antibody - Aminoterminal end (ab71728) at 1/100 dilution + Y79 cell lysate at 12.5 µg Predicted band size : 94 kDa Observed band size : 104 kDa ( why is the actual band size different from the predicted?) Additional bands at : 40 kDa,50 kDa. We are unsure as to the identity of these extra bands.
References for Anti-EPS15R antibody - Aminoterminal end (ab71728)
has not yet been referenced specifically in any publications.
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